2024-03-29T08:40:22Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1632212022-06-07T11:20:18Zcom_10261_34com_10261_5col_10261_287
A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes
Roy, D.
Athey, T.B.T.
Auger, J.P.
Goyette-Desjardins, G.
Van Calsteren, M.R.
Takamatsu, D.
Okura, M.
Teatero, S.
Alcorlo, Martín
Hermoso, Juan A.
Segura, Marta
Gottschalk, M.
Fittipaldi, N.
Ministerio de Economía y Competitividad (España)
The capsular polysaccharide (CPS) is the major virulence factor of the emerging zoonotic pathogen Streptococcus suis. CPS differences are also the basis for serological differentiation of the species into 29 serotypes. Serotypes 2 and 1/2, which possess identical gene content in their cps loci, express CPSs that differ only by substitution of galactose (Gal) by N-acetylgalactosamine (GalNAc) in the CPS side chain. The same sugar substitution differentiates the CPS of serotypes 14 and 1, whose cps loci are also identical in gene content. Here, using mutagenesis, CPS structural analysis, and protein structure modeling, we report that a single amino acid polymorphism in the glycosyltransferase CpsK defines the enzyme substrate predilection for Gal or GalNAc and therefore determines CPS composition, structure, and strain serotype. We also show that the different CPS structures have similar antiphagocytic properties and that serotype switching has limited impact on the virulence of S. suis.
2018-04-05T16:03:43Z
2018-04-05T16:03:43Z
2017
2018-04-05T16:03:43Z
artículo
Scientific Reports 7 (2017)
http://hdl.handle.net/10261/163221
10.1038/s41598-017-04403-3
http://dx.doi.org/10.13039/501100003329
28642597
eng
Publisher's version
Sí
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-59389-P
openAccess
Nature Publishing Group