2024-03-29T15:49:08Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1603192020-12-12T16:54:17Zcom_10261_41com_10261_1col_10261_294
The tumorigenic properties of Drosophila epithelial cells mutant for lethal giant larvae
Calleja, Manuel
Morata, Ginés
Casanova, Jordi
Ministerio de Economía y Competitividad (España)
Fundación Ramón Areces
Generalitat de Catalunya
Mutations in Drosophila tumor suppressor genes (TSGs) lead to the formation of invasive tumors in the brain and imaginal discs. Results: Here we studied the tumorigenic properties of imaginal discs mutant for the TSG gene lethal giant larvae (lgl). lgl mutant cells display the characteristic features of mammalian tumor cells: they can proliferate indefinitely, induce additional tracheogenesis (an insect counterpart of vasculogenesis) and invade neighboring tissues. Lgl mutant tissues exhibit high apoptotic levels, which lead to the activation of the Jun-N-Terminal Kinase (JNK) pathway. We propose that JNK is a key factor in the acquisition of these tumorigenic properties; it promotes cell proliferation and induces high levels of Mmp1 and confers tumor cells capacity to invade wild-type tissue. Noteworthy, lgl RNAi-mediated down-regulation does not produce similar transformations in the central nervous system (CNS), thereby indicating a fundamental difference between the cells of developing imaginal discs and those of differentiated organs. We discuss these results in the light of the ¿single big-hit origin¿ of some human pediatric or developmental cancers. Conclusions: Down-regulation of lgl in imaginal discs is sufficient to enhance tracheogenesis and to promote invasion and colonization of other larval structures including the CNS.
2018-02-08T11:11:25Z
2018-02-08T11:11:25Z
2016-07-15
2018-02-08T11:11:25Z
artículo
Developmental Dynamics 245: 834- 843 (2016)
http://hdl.handle.net/10261/160319
10.1002/dvdy.24420
http://dx.doi.org/10.13039/501100002809
http://dx.doi.org/10.13039/100008054
http://dx.doi.org/10.13039/501100003329
eng
Postprint
https://www.doi.org/10.1002/dvdy.24420
Sí
closedAccess
John Wiley & Sons