2024-03-29T15:16:21Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1492002021-12-28T15:33:36Zcom_10261_131com_10261_2col_10261_384
Gastrointestinal Endogenous Protein-Derived Bioactive Peptides: An in Vitro Study of Their Gut Modulatory Potential
Dave, Lakshmi A.
Hayes, María
Mora, Leticia
Montoya, Carlos A.
Moughan, Paul J.
Rutherfurd, Shane M.
Consejo Superior de Investigaciones Científicas (España)
A recently proposed paradigm suggests that, like their dietary counterparts, digestion of gastrointestinal endogenous proteins (GEP) may also produce bioactive peptides. With an aim to test this hypothesis, <i>in vitro</i> digests of four GEP namely; trypsin (TRYP), lysozyme (LYS), mucin (MUC), serum albumin (SA) and a dietary protein chicken albumin (CA) were screened for their angiotensin-I converting (ACE-I), renin, platelet-activating factor-acetylhydrolase (PAF-AH) and dipeptidyl peptidase-IV inhibitory (DPP-IV) and antioxidant potential following simulated <i>in vitro</i> gastrointestinal digestion. Further, the resultant small intestinal digests were enriched to obtain peptides between 3–10 kDa in size. All <i>in vitro</i> digests of the four GEP were found to inhibit ACE-I compared to the positive control captopril when assayed at a concentration of 1 mg/mL, while the LYS < 3-kDa permeate fraction inhibited renin by 40% (±1.79%). The LYS < 10-kDa fraction inhibited PAF-AH by 39% (±4.34%), and the SA < 3-kDa fraction inhibited DPP-IV by 45% (±1.24%). The MUC < 3-kDa fraction had an ABTS-inhibition antioxidant activity of 150 (±24.79) µM trolox equivalent and the LYS < 10-kDa fraction inhibited 2,2-Diphenyl-1-picrylhydrazyl (DPPH) by 54% (±1.62%). Moreover, over 190 peptide-sequences were identified from the bioactive GEP fractions. The findings of the present study indicate that GEP are a significant source of bioactive peptides which may influence gut function.
2017-05-06T05:22:21Z
2017-05-06T05:22:21Z
2016-04-01
2017-05-06T05:22:21Z
artículo
International Journal of Molecular Sciences 17 (4): 482 (2016)
http://hdl.handle.net/10261/149200
10.3390/ijms17040482
http://dx.doi.org/10.13039/501100003339
27043546
Publisher's version
http://doi.org/10.3390/ijms17040482
Sí
http://creativecommons.org/licenses/by/4.0/
openAccess
Multidisciplinary Digital Publishing Institute