2024-03-29T23:27:57Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1491882021-12-28T15:34:36Zcom_10261_72com_10261_6col_10261_325
Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster
Baenas, Nieves
Piegholdt, Stefanie
Schloesser, Anke
Moreno, Diego A.
García-Viguera, Cristina
Rimbach, Gerald
Wagner, Anika E.
Consejo Superior de Investigaciones Científicas (España)
We used <i>Drosophila melanogaster</i> as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (<i>Raphanus sativus</i> cv. Rambo), a <i>Brassicaceae</i> species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that <i>Drosophila melanogaster</i> takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of <i>spargel</i> expression, the <i>Drosophila</i> homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase <i>in vitro</i>. Overall, we show that the consumption of radish sprouts affects energy metabolism in <i>Drosophila melanogaster</i> which is reflected by lower glucose levels and an increased expression of <i>spargel</i>, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus.
2017-05-06T05:21:26Z
2017-05-06T05:21:26Z
2016-02-18
2017-05-06T05:21:27Z
artículo
International Journal of Molecular Sciences 17 (2): 251 (2016)
http://hdl.handle.net/10261/149188
10.3390/ijms17020251
http://dx.doi.org/10.13039/501100003339
26901196
Publisher's version
http://doi.org/10.3390/ijms17020251
Sí
http://creativecommons.org/licenses/by/4.0/
openAccess
Multidisciplinary Digital Publishing Institute