2024-03-28T08:30:52Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1441862022-11-23T11:36:14Zcom_10261_108com_10261_8col_10261_361
Eurasian wild boar response to skin-testing with mycobacterial and non mycobacterial antigens
Jaroso, Raquel
Vicente, Joaquín
Fernández de Mera, Isabel G.
Aranaz, Alicia
Gortázar, Christian
European Commission
Fundación Botín
Ministerio de Educación y Ciencia (España)
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Junta de Comunidades de Castilla-La Mancha
Skin test
Sus scrofa
Phytohaemagglutinin
Diagnostic accuracy
Cellular immunity
Bovine tuberculosis
Eurasian wild boar (Sus scrofa) are able to maintain bovine tuberculosis (bTB) in the wild
and are most probably able to transmit the disease to other species, thus acting as a true
wildlife reservoir. Translocation of wild boar is a common practice in European countries.
Therefore, identifying effective tools for bTB diagnosis in living wild boar is crucial for
the implementation of control measures. We describe for the first time the sex and origin
related differences in the skin-test response to mycobacterial antigens (bPPD and aPPD)
and to a non-mycobacterial antigen (PHA, a plant derived mitogen) in wild and farmed wild
boar, and used a small sample of known M. bovis infected wild boar to establish whether
skin-testing is an option for bTB diagnosis in living wild boar. The highest skinfold increase response was detected at the PHA injection site, evidencing that the PHA test could be useful in monitoring cell mediated immunity (CMI) in wild boar populations. A clear ageincreasing trend of the PHA response indicated that age should be taken into account when measuring CMI in wild boar. Origin related differences in the response against mycobacterial antigens could reflect differential exposure to mycobacterial antigens. Skin testing in BCG immunized wild boar showed low sensitivity (43-57%), while the sensitivity was good in the culture positive controls (75-100%), depending on the reading criterion. Specificity improved when the criterion was a response to bPPD larger than 2mm and bPPD response larger than aPPD response (77%). Although a limited sample, our results indicated the potential of skin test as a bTB diagnostic tool in Eurasian wild boar. However, handling of wild boar is dangerous, specificity is low, and more effort is needed in order to define the sensitivity of this technique.
2017-02-17T11:20:51Z
2017-02-17T11:20:51Z
2010
2017-02-17T11:20:51Z
artículo
Preventive Veterinary Medicine 96(3-4): 211-217 (2010)
http://hdl.handle.net/10261/144186
10.1016/j.prevetmed.2010.06.011
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100007273
http://dx.doi.org/10.13039/501100006373
http://dx.doi.org/10.13039/501100011698
eng
Sí
info:eu-repo/grantAgreement/EC/FP7/212414
closedAccess
Elsevier