2024-03-28T20:21:19Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1304602021-03-18T16:55:29Zcom_10261_63com_10261_6col_10261_442
Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms
Ramos, M.
Mateos, Iván
Saro, Cristina
Díaz, A.
Ranilla, María José
Rumen content treatment
Methane
Volatile fatty acids
Trabajo presentado al: Joint Annual Meeting. ADSA-ASAS.( Orlando, Florida, USA, 12-16 julio 2015. T486
The characteristics of the inoculum are recognized as one of the most
relevant factors infiuencing the results o fin vitro fennentations in batch
cultures of ruminal microorganisms. Four rumen-fistulated sheep fed
a 66:34 alfalfa hay:concentrate diet were used as donors to investigate
the effect ofrumen contents' processing on in vitro methane (CH4) and
volatile fatty acid (VFA) production from 3 substrates ofvariable composition. Rumen contents were sampled from each individual sheep and
subjected to the following treatments: SQ) squeezed through 4 layers of
cheesecloth; FIL) SQ treatment and further filtration through a 100-µm
nylon cloth; STO) treated with a Stomacher for 3 min at 230 rev min-1
and followed by SQ. The resulting fiuids were used as inoculum for
batch cultures containing alfalfa hay, concentrate, or a 50:50 mixtnre
ofboth feeds. Cultures were incubated at 39°C for 8 and 24 h, and CH4
and VF A production was measured. There were no treatment x substrate interactions (P > 0.05) for any variable at any incubation time, excepting
for the molar proportion of acetate at 24 h (P = 0.019). The method of
processing the rumen contents did not affect (P > 0.05) eithertotal VFA
and CH4 production or molar proportions of individual VF A at any time.
At both incubation times, increasing the amount of concentrate in the
substrate increased CH4 production (P < 0.001, quadratic) and molar
proportion ofbutyrate (P < 0.001, linear), but decreased acetate proportion (P < 0.001, quadratic) without affecting (P > 0.05) proportions of
propionate. Whereas total VFA production was linearly decreased (P
= 0.007) by increased amounts of concentrate in the substrate at 8 h
ofincubation, it was quadratically increased (P < 0.001) after 24 h of
incubation. There were clear differences in CH4 and VFA production
among inocula from different sheep, which persisted across substrates.
The results show that the tested methods ofprocessing rumen contents
did not affect in vitro fermentation characteristics of good quality substrates, but studies ana!yzing their possible infiuence on fermentation
of!ow-quality substrates are required
2016-03-28T12:36:18Z
2016-03-28T12:36:18Z
2015
póster de congreso
Journal of Animal Science 93 (Suppl. s3/J) 484 (2015)
http://hdl.handle.net/10261/130460
1525-3163
eng
http://www.jtmtg.org/jam/2015/
Sí
closedAccess
American Society of Animal Science