2024-03-28T19:35:25Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1242992021-06-14T11:25:07Zcom_10261_79com_10261_1col_10261_332
Cyclooxygenase-2 and prostaglandin E<inf>2</inf> signaling through prostaglandin receptor EP- 2 favor the development of myocarditis during acute trypanosoma cruzi infection
Guerrero, Néstor A.
Camacho, Mercedes
Vila, Luis
Íñiguez, Miguel Ángel
Chillón-Marinas, Carlos
Cuervo, Henar
Poveda, Cristina
Fresno, Manuel
Gironès, Núria
Ministerio de Asuntos Exteriores y Cooperación (España)
Universidad Autónoma de Madrid
Instituto de Salud Carlos III
Red de Investigación Cooperativa en Enfermedades Tropicales (España)
Fundación Ramón Areces
Ministerio de Sanidad, Servicios Sociales e Igualdad (España)
Ministerio de Ciencia e Innovación (España)
Inflammation plays an important role in the pathophysiology of Chagas disease, caused by Trypanosoma cruzi. Prostanoids are regulators of homeostasis and inflammation and are produced mainly by myeloid cells, being cyclooxygenases, COX-1 and COX-2, the key enzymes in their biosynthesis from arachidonic acid (AA). Here, we have investigated the expression of enzymes involved in AA metabolism during T. cruzi infection. Our results show an increase in the expression of several of these enzymes in acute T. cruzi infected heart. Interestingly, COX-2 was expressed by CD68<sup>+</sup> myeloid heart-infiltrating cells. In addition, infiltrating myeloid CD11b<sup>+</sup>Ly6G<sup>-</sup> cells purified from infected heart tissue express COX-2 and produce prostaglandin E<inf>2</inf> (PGE<inf>2</inf>) ex vivo. T. cruzi infections in COX-2 or PGE<inf>2</inf>- dependent prostaglandin receptor EP-2 deficient mice indicate that both, COX-2 and EP-2 signaling contribute significantly to the heart leukocyte infiltration and to the release of chemokines and inflammatory cytokines in the heart of T. cruzi infected mice. In conclusion, COX-2 plays a detrimental role in acute Chagas disease myocarditis and points to COX-2 as a potential target for immune intervention.
2015-08-25
2015-11-02T12:36:07Z
artículo
PLoS Neglected Tropical Diseases 9(8): e0004025 (2015)
http://hdl.handle.net/10261/124299
10.1371/journal.pntd.0004025
http://dx.doi.org/10.13039/501100003767
http://dx.doi.org/10.13039/501100004593
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/100008054
http://dx.doi.org/10.13039/501100003751
http://dx.doi.org/10.13039/501100004837
26485277
eng
Publisher's version
Sí
openAccess
Public Library of Science