2024-03-28T11:41:17Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1156452018-10-03T07:52:34Zcom_10261_9676com_10261_8col_10261_9677
RNAi reveals the key role of Nervana 1 in cockroach oogenesis and embryo development
Irles, Paula
Silva-Torres, Fernanda A.
Piulachs, Maria-Dolors
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas (España)
Comisión Nacional de Investigación Científica y Tecnológica (Chile)
Fundação de Amparo à Pesquisa do Estado de São Paulo
Embryogenesis
Patency
Blattella germanica
Drosophila
Panoistic ovary
Ion pump
Na+, K+-ATPases is a heterodimer protein consisting of α- and β-subunits that control the ion transport through cell membranes. In insects the β-subunit of the Na+, K+-ATPase, known as Nervana, was characterized as a nervous system-specific glycoprotein antigen from adult Drosophila melanogaster heads. Nervana is expressed ubiquitously in all insect tissues, and in epithelial cells appeared located in a basolateral position as part of the septate junctions. Herein we study two Nervana isoforms from Blattella germanica, a cockroach species with panoistic ovaries. The sequencing and the phylogenetic analysis results suggest that these two isoforms are orthologs of D. melanogaster Nervana 1 and Nervana 2, respectively. Nervana 1 is highly expressed in the ovary of B. germanica, and depleting its expression results in changes in oocyte shape that do not impair oviposition. However, the resulting embryos show different defects and never hatch. These findings highlight the importance of this type of membrane pump in insect oogenesis as well as in embryo development, and its possible regulation by juvenile hormone. © 2012 Elsevier Ltd.
2015-05-22T10:50:04Z
2015-05-22T10:50:04Z
2013-02
2015-05-22T10:50:04Z
artículo
Insect Biochemistry and Molecular Biology 43(2): 178-188 (2013)
http://hdl.handle.net/10261/115645
10.1016/j.ibmb.2012.12.003
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100002848
http://dx.doi.org/10.13039/501100001807
eng
Postprint
http://dx.doi.org/10.1016/j.ibmb.2012.12.003
Sí
openAccess
Elsevier