2024-03-28T08:20:04Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1139092019-03-22T11:34:59Zcom_10261_41com_10261_1col_10261_294
Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): A rigid molecular scaffold
Krätzner, Ralph
Debreczeny, Judith E.
Pape, Thomas
Schneider, Thomas R.
Wentzel, Alexander
Kolmar, Harald
Sheldrick, George M.
Usón, Isabel
knottins
inhibitors
EETI-II
molecular scaffolds
Cystine knots
The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold. © 2005 International Union of Crystallography - all rights reserved.
2015-04-21T09:51:36Z
2015-04-21T09:51:36Z
2005-09
2015-04-21T09:51:36Z
artículo
Acta Crystallographica Section D: Biological Crystallography 61(9): 1255-1262 (2005)
http://hdl.handle.net/10261/113909
10.1107/S0907444905021207
eng
Publisher's version
http://dx.doi.org/10.1107/S0907444905021207
openAccess
Blackwell Publishing