2024-03-28T18:19:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1111752021-11-22T12:59:43Zcom_10261_41com_10261_1col_10261_294
Structure and interaction with phospholipids of a prokaryotic lipoxygenase from Pseudomonas aeruginosa
Garreta, Albert
García-Fernández, Queralt
Fita, Ignacio
Carpena, Xavi
Membrane interaction
Enzyme mechanisms
Host-pathogen interactions
Lipid peroxidation
Protein-phospholipid complexes
Albert Garreta et al.
Lipoxygenases (LOXs), which are essential in eukaryotes, have no confirmed function in prokaryotes that are devoid of polyunsaturated fatty acids. The structure of a secretable LOX from Pseudomonas aeruginosa (Pa-LOX), the first available from a prokaryote, presents significant differences with respect to eukaryotic LOXs, including a cluster of helices acting as a lid to the active center. The mobility of the lid and the structural variability of the N-terminal region of Pa-LOX was confirmed by comparing 2 crystal forms. The binding pocket contains a phosphatidylethanolamine phospholipid with branches of 18 (sn-1) and 14/16 (sn-2) carbon atoms in length. Carbon atoms from the sn-1 chain approach the catalytic iron in a manner that sheds light on how the enzymatic reaction might proceed. The findings in these studies suggest that Pa-LOX has the capacity to extract and modify unsaturated phospholipids from eukaryotic membranes, allowing this LOX to play a role in the interaction of P. Aeruginosa with host cells.Garreta, A., Val-Moraes, S. P., García-Fernández, Q., Montserrat Busquets, C. J., Oliver, A., Ortiz, A., Gaffney, B. J., Fita, I., Manresa, A., Carpena, X. Structure and interaction with phospholipids of a prokaryotic lipoxygenase from Pseudomonas aeruginosa. © FASEB.
2015-02-24T14:24:48Z
2015-02-24T14:24:48Z
2013-12
2015-02-24T14:24:49Z
artículo
FASEB Journal 27(12): 4811-4821 (2013)
http://hdl.handle.net/10261/111175
10.1096/fj.13-235952
23985801
eng
http://dx.doi.org/10.1096/fj.13-235952
closedAccess
Federation of American Societies for Experimental Biology