2024-03-19T08:53:38Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1021152021-06-11T07:45:33Zcom_10261_115com_10261_3col_10261_368
"Michael" nanocarriers mimicking transient-binding disordered proteins
Sánchez-Sánchez, Ana
Akbari, Somayeh
Etxeberria, Agustín
Arbe, Arantxa
Gasser, Urs
Moreno Segurado, Ángel J.
Colmenero de León, Juan
Pomposo, José A.
European Commission
Ministerio de Economía y Competitividad (España)
Eusko Jaurlaritza
We report herein a very efficient synthesis strategy for the construction of artificial transient-binding protein-mimic nano-objects. Michael addition-mediated multidirectional self-assembly of individual polymeric chains at r.t. leads to >Michael> nanocarriers that in solution resemble disordered multidomain proteins, as revealed by a combination of small angle neutron scattering measurements and coarse-grained molecular dynamics simulation results, whereas in the dry state adopt a collapsed, globular morphology, as observed by transmission electron microscopy. This extended-to-compact morphology transition taking place upon solvent removal is of paramount importance, among other applications, for the construction of efficient biosensors based on immobilized protein-mimic nano-objects and for the development of transient vitamin-binding systems. As a proof of concept, we show the controlled delivery of vitamin B9 from these novel transient-binding nanocarriers. © 2013 American Chemical Society.
2014-09-15T13:07:37Z
2014-09-15T13:07:37Z
2013
2014-09-15T13:07:37Z
artículo
ACS Macro Letters 2(6): 491-495 (2013)
http://hdl.handle.net/10261/102115
10.1021/mz400173c
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100003086
eng
Postprint
http://dx.doi.org/10.1021/mz400173c
info:eu-repo/grantAgreement/EC/FP7/283883
openAccess
American Chemical Society