2024-03-29T14:18:02Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1001652022-05-31T11:12:12Zcom_10261_63com_10261_6col_10261_316
Comparison of three immunological diagnostic tests for the detection of avian tuberculosis in naturally infected red deer (Cervus elaphus)
Fernández, J. G.
Fernández de Mera, Isabel G.
Reyes, L. E.
Ferreras, Mª del Carmen
Pérez Pérez, Valentín
Gortázar, C.
Fernández Fernández, M.
García Marín, Juan Francisco
Enzyme-linked immunosorbent assay
Avian tuberculosis
Mycobacterium avium
Interferon-gamma
Deer
6 páginas, 1 figura, 3 tablas.
Cases of tuberculosis due to Mycobacterium avium subsp. avium in 52 adult red deer (Cervus elaphus) from a farm were studied using different diagnostic techniques. Immunological probes consisted of the comparative cervical tuberculin (CCT) skin test, the interferon-γ (IFN-γ) assay, and 2 enzyme-linked immunosorbent assays (ELISAs) employing either avian purified protein derivatives or protoplasmatic antigen (PPA-3) as antigens. Three of the animals were euthanized due to severe weakness, loss of weight, and emaciation. Macroscopically, the 3 animals showed tuberculous lesions located mainly in lymph nodes of the digestive system and small intestine but also in other organs and lymph nodes. Polymerase chain reaction was carried out on samples from the 3 deer using primers to detect IS901, IS900, and IS6110, specific for Mycobacterium avium subsp. avium, Mycobacterium avium subsp. paratuberculosis, and Mycobacterium tuberculosis complex, including Mycobacterium bovis, respectively. The last 2 agents cause pathologies very similar to avian tuberculosis in deer. The 3 deer were strongly positive by both ELISAs, slightly positive by the IFN-c test, and 1 of 2 was positive by the CCT test. As with domestic ruminants, ELISA could detect deer in an advanced stage of infection, with large numbers of mycobacteria.
2014-07-18T11:22:32Z
2014-07-18T11:22:32Z
2009
2014-07-18T11:22:32Z
artículo
Journal of Veterinary Diagnostic Investigation 21: 102- 107 (2009)
1040-6387
http://hdl.handle.net/10261/100165
10.1177/104063870902100115
eng
http://dx.doi.org/10.1177/104063870902100115
closedAccess
American Association of Veterinary Laboratory Diagnosticians