2024-03-28T09:50:59Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/790132021-06-07T11:57:03Zcom_10261_22com_10261_1col_10261_275
In situ hybridization analyses of Na, K-ATPase α-subunit expression during early larval development of Artemia franciscana
Escalante, Ricardo
García-Sáez, Alberto
Sastre, Leandro
The spatial pattern of expression of the mRNA encoded by the Na,K-ATPase α-subunit cDNA clone pArATNa136 was determined by in situ hybridization of first, second, and third instar Artemia franciscana larvae. This mRNA was expressed at high levels in the salt gland, the antennal gland, and the end of the midgut, which are the three main osmoregulatory organs in Artemia at these stages of development. The pattern of expression was similar at the three stages of development analyzed, although the level of expression increased during development, especially in the salt and antennal glands. The expression of the mRNA coding for another Na,K-ATPase α-subunit isoform, the proposed α2-isoform, was also determined and was shown to be limited to the salt gland. These results suggest that the done pArATNa136 codes for the biochemically defined α1-isoform of the Na,K-ATPase α-subunit and reinforce the importance of this isoform in osmoregulation at the three larval stages studied. The α2-isoform may also be involved in osmoregulation during the first stages of larval development.
Supported by grant PB92-0076 from the Dirección General de Investigación Científica y Técnica and by a pre-doctoral fellowship from the Comunidad Autónoma de Madrid (RE).
Peer Reviewed
2013-07-03T12:24:00Z
2013-07-03T12:24:00Z
1995
2013-07-03T12:24:00Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1177/43.4.7897181
issn: 0022-1554
e-issn: 1551-5044
Journal of Histochemistry and Cytochemistry 43(4): 391-399 (1995)
http://hdl.handle.net/10261/79013
10.1177/43.4.7897181
en
none
Sage Publications