2024-03-28T08:23:11Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/653412016-02-17T11:32:03Zcom_10261_47com_10261_8col_10261_300
Identification of IL-34 in teleost fish: differential expression of rainbow trout IL-34, MCSF1 and MCSF2, ligands of the MCSF receptor
Wang, T.
Kono, Tomoya
Monte, Milena M.
Kuse, Haruka
Costa, M. M.
Korenaga, Hiroki
Maehr, Tanja
Husain, Mansourah
Sakai, Masahiro
Secombes, Christopher J.
Teleost fish
IL-34
MCSF
MCSFR
Gene cloning
Differential expression
11 páginas, 8 figuras, 2 tablas
The mononuclear phagocyte system is composed of monocytes, macrophages and dendritic cells and has crucial roles in inflammation, autoimmunity, infection, cancer, organ transplantation and in maintaining organismal homeostasis. Interleukin-34 (IL-34) and macrophage colony stimulating factor (MCSF), both signalling through the MCSF receptor, regulate the mononuclear phagocyte system. A single IL-34 and MCSF gene are present in tetrapods. Two types of MCSF exist in teleost fish which is resulted from teleost-wide whole genome duplication. In this report, we first identified and sequence analysed six IL-34 genes in five teleost fish, rainbow trout, fugu, Atlantic salmon, catfish and zebrafish. The fish IL-34 molecules had a higher identity within fish group but low identities to IL-34s from birds (27.2–33.8%) and mammals (22.2–31.4%). However, they grouped with tetrapod IL-34 molecules in phylogenetic tree analysis, had a similar 7 exon/6 intron gene organisation, and genes in the IL-34 loci were syntenically conserved. In addition, the regions of the four main helices, along with a critical N-glycosylation site were well conserved. Taken together these data suggest that the teleost IL-34 genes described in this report are orthologues of tetrapod IL-34.
Comparative expression study of the three trout MCSFR ligands revealed that IL-34, MCSF1 and MCSF2 are differentially expressed in tissues and cell lines. The expression of MCSF1 and MCSF2 showed great variance in different tissues and cell lines, suggesting a role in the differentiation and maintenance of specific macrophage lineages in specific locations. The relatively high levels of IL-34 expression across different tissues suggests a homeostatic role of IL-34 for the macrophage lineage in fish. One striking observation in the present study was the lack of induction of MCSF1 and MCSF2 expression but the quick induction of IL-34 expression by PAMPs and inflammatory cytokines in cell lines and primary head kidney macrophages in rainbow trout. In a parasitic proliferative kidney disease (PKD) model, the expression of IL-34 but not the dominant MCSF2 was affected by PKD, suggesting an involvement of macrophage function in this disease model. Thus IL-34 expression is sensitive to inflammatory stimuli and may regulate macrophage biology once up-regulated.
TW received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). TK was supported financially by the Program “Improvement of Research Environment for Young Researchers” from the Japanese Ministry of Education, Culture, Sports, Science and Technology, a Grant-in-Aid for Young Scientists (23780199) and a grant for Scientific Research on Priority Areas from the University of Miyazaki. MMC thanks the Consejo Superior de Investigaciones Científicas (CSIC, Spain) and the Xunta de Galicia for her “Ángeles Alvariño” postdoctoral contract. Thanks to Dr. Jason Holland (Scottish Fish Immunology Research Centre, University of Aberdeen) for supplying of the PKD samples.
Peer reviewed
2013-01-29T09:18:33Z
2013-01-29T09:18:33Z
2012
artículo
http://purl.org/coar/resource_type/c_6501
Molecular Immunology 63(4): 398-409 (2012)
0161-5890
http://hdl.handle.net/10261/65341
10.1016/j.molimm.2012.09.008
1872-9142
en
http://dx.doi.org/10.1016/j.molimm.2012.09.008
open
Elsevier