2024-03-28T15:07:40Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/63212021-12-28T16:01:22Zcom_10261_86com_10261_1col_10261_339
Human pregnane X receptor is expressed in breast carcinomas, potential heterodimers formation between hPXR and RXR-alpha
Conde, Isabel
Lobo, María del Val Toledo
Zamora, Javier
Pérez, Julio
González, Francisco J.
Alba, Emilio
Fraile, Benito
Paniagua, Ricardo
Arenas, María I.
Human pregnane X receptor
hPXR
SXR
Heterodimers
Breast cancer
14 p.-5 fig.-5 tab.
[Background] The human pregnane X receptor (hPXR) is an orphan nuclear receptor that induces transcription of response elements present in steroid-inducible cytochrome P-450 gene promoters. This activation requires the participation of retinoid X receptors (RXRs), needed partners of hPXR to form heterodimers. We have investigated the expression of hPXR and RXRs in normal, premalignant, and malignant breast tissues, in order to determine whether their expression profile in localized infiltrative breast cancer is associated with an increased risk of recurrent disease.
[Methods] Breast samples from 99 patients including benign breast diseases, in situ and infiltrative carcinomas were processed for immunohistochemistry and Western-blot analysis.
[Results] Cancer cells from patients that developed recurrent disease showed a high cytoplasmic location of both hPXR isoforms. Only the infiltrative carcinomas that relapsed before 48 months showed nuclear location of hPXR isoform 2. This location was associated with the nuclear immunoexpression of RXR-alpha.
[Conclusions] Breast cancer cells can express both variants 1 and 2 of hPXR. Infiltrative carcinomas that recurred showed a nuclear location of both hPXR and RXR-alpha; therefore, the overexpression and the subcellular location changes of hPXR could be considered as a potential new prognostic indicator.
Peer reviewed
2008-08-04T07:14:00Z
2008-08-04T07:14:00Z
2008-06-19
artículo
http://purl.org/coar/resource_type/c_6501
BMC Cancer 2008, 8:174
1471-2407
http://hdl.handle.net/10261/6321
10.1186/1471-2407-8-174
18565212
en
Publisher’s version
http://dx.doi.org/10.1186/1471-2407-8-174
open
2248428 bytes
application/pdf
BioMed Central
http://dx.doi.org/10.1186/1471-2407-8-174