2024-03-30T03:26:23Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/623472021-12-27T16:43:44Zcom_10261_54com_10261_1col_10261_307
Prenatal stress induces long-term effects in cell turnover in the hippocampus-hypothalamus-pituitary axis in adult male rats
Baquedano, E.
García-Cáceres, C.
Diz-Chaves, Yolanda
Lagunas, Natalia
Calmarza-Font, Isabel
Azcoitia, I.
García-Segura, Luis M.
Argente, Jesús
Chowen, Julie A.
Frago, L. M.
Subchronic gestational stress leads to permanent modifications in the hippocampus-hypothalamus-pituitary-adrenal axis of offspring probably due to the increase in circulating glucocorticoids known to affect prenatal programming. The aim of this study was to investigate whether cell turnover is affected in the hippocampus-hypothalamus-pituitary axis by subchronic prenatal stress and the intracellular mechanisms involved. Restraint stress was performed in pregnant rats during the last week of gestation (45 minutes; 3 times/day). Only male offspring were used for this study and were sacrificed at 6 months of age. In prenatally stressed adults a decrease in markers of cell death and proliferation was observed in the hippocampus, hypothalamus and pituitary. This was associated with an increase in insulin-like growth factor-I mRNA levels, phosphorylation of CREB and calpastatin levels and inhibition of calpain -2 and caspase -8 activation. Levels of the anti-apoptotic protein Bcl-2 were increased and levels of the pro-apoptotic factor p53 were reduced. In conclusion, prenatal restraint stress induces a long-term decrease in cell turnover in the hippocampus-hypothalamus-pituitary axis that might be at least partly mediated by an autocrine-paracrine IGF-I effect. These changes could condition the response of this axis to future physiological and pathophysiological situations. © 2011 Baquedano et al.
Peer Reviewed
2012-12-10T15:59:21Z
2012-12-10T15:59:21Z
2011
2012-12-10T15:59:21Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1371/journal.pone.0027549
issn: 1932-6203
PLoS ONE 6 (2011)
http://hdl.handle.net/10261/62347
10.1371/journal.pone.0027549
22096592
en
Publisher’s version
open
Public Library of Science