2024-03-28T17:20:50Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/609262012-11-22T23:00:00Zcom_10261_47com_10261_8col_10261_300
A HPLC method for specific determination ofα-amylase and glucoamylase in complex enzymatic preparations
Franco, José M.
Murado García, Miguel Anxo
Siso, M. I. G.
Mirón, Jesús
González Fernández, Pilar
Column liquid chromatography
Glucose, maltose and maltotriose
α-Amylase and glucoamylase
Microfungal cultures
5 páginas, 4 figuras, 4 tablas
In the hydrolysis of soluble starch by mixtures of α-amylase and glucoamylase, the ratios maltose/glucose and maltoriose/glucose linearly depend, over a wide range, on the relation between both enzymes and are independent on the activity level of the enzymatic preparation. HPLC determination of hydrolysis products (glucose, maltose and maltotriose) of soluble starch by mixtures of these enzymes, after incubation under controlled conditions, is a rapid method for the evaluation of the relative levels of each enzyme in the mixtures. The method, first developed using pure commercial amylases, is applied, with consistent results, to cell free media ofAspergillus niger cultures on a glycogen-rich effluent.
CICYT (project ID 87060).
Peer reviewed
2012-11-23T08:19:38Z
2012-11-23T08:19:38Z
1989
artículo
http://purl.org/coar/resource_type/c_6501
Chromatographia 27(7-8): 328-332 (1989)
0009-5893
http://hdl.handle.net/10261/60926
10.1007/BF02321279
1612-1112
en
http://dx.doi.org/10.1007/BF02321279
none
Springer