2024-03-28T23:58:53Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/579592018-09-13T09:06:11Zcom_10261_15com_10261_6col_10261_268
Growth of phototrophic biofilms from limestone monuments under laboratory conditions
Miller, A. Z.
Laiz Trobajo, L.
Dionísio, A.
Macedo, M. F.
Sáiz-Jiménez, Cesáreo
Ministério da Ciência, Tecnologia e Ensino Superior (Portugal)
Fundação para a Ciência e a Tecnologia (Portugal)
European Commission
In the current study, five phototrophic biofilms from different Southern Europe limestone monuments were characterised by molecular techniques and cultivated under laboratory conditions. Phototrophic biofilms were collected from Orologio Tower in Martano (Italy), Santa Clara-a-Velha Monastery and Ajuda National Palace, both in Portugal, and Seville and Granada Cathedrals from Spain. The biofilms were grown under laboratory conditions and periodically sampled in order to monitor their evolution over a three-month period. Prokaryotic communities from natural samples and cultivated biofilms were monitored using denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments in conjunction with clone sequencing and phylogenetic analysis. DNA-based molecular analysis of 16S rRNA gene fragments from the natural green biofilms revealed complex and different communities composition with respect to phototrophic microorganisms. The biofilms from Orologio Tower (Martano, Italy) and Santa Clara-a-Velha Monastery (Coimbra, Portugal) were dominated by the microalga Chlorella. The cyanobacterium Chroococcidiopsis was the dominating genus from Ajuda National Palace biofilm (Lisbon, Portugal). The biofilms from Seville and Granada Cathedrals (Spain) were both dominated by the cyanobacterium Pleurocapsa. The DGGE analysis of the cultivated biofilms showed that the communities developed differently in terms of species establishment and community composition during the three-month incubation period. The biofilm culture from Coimbra (Portugal) showed a remarkable stability of the microbial components of the natural community in laboratory conditions. With this work, a multiple-species community assemblage was obtained for further stone colonisation experiments. © 2009 Elsevier Ltd. All rights reserved.
This work was supported by the Ministério da Ciência, Tecnologia e Ensino Superior, Portugal, with a doctoral grant (SFRH/BD/ 21481/2005) and partially financed by the CEPGIST FCT subproject DECASTONE. The work also received support from Programa de Fianciamento Plurianual da Unidade de Investigaçao da FCT, financed by European Union FEDER and national budget of Portuguese Republic. The projects TCP CSD2007-00058 and 2007PT0041 are acknowledged.
Peer Reviewed
2012-10-15T07:28:59Z
2012-10-15T07:28:59Z
2009
2012-10-15T07:28:59Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1016/j.ibiod.2009.04.004
issn: 0964-8305
International Biodeterioration & Biodegradation 63(7): 860- 867 (2009)
http://hdl.handle.net/10261/57959
10.1016/j.ibiod.2009.04.004
http://dx.doi.org/10.13039/501100006111
http://dx.doi.org/10.13039/501100001871
http://dx.doi.org/10.13039/501100000780
en
none
Elsevier