2024-03-29T01:38:30Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/508542020-10-20T11:19:20Zcom_10261_86com_10261_1col_10261_339
Structural Framework for the Modulation of the Activity of the Hybrid Antibiotic Peptide Cecropin A-Melittin [CA(1-7)M(2-9)] by N(ε) -Lysine Trimethylation
Díaz, Dolores
García de la Torre, Beatriz
Fernández-Reyes, María
Rivas, Luis
Andreu, David
Jiménez-Barbero, Jesús
Antimicrobial peptides
Cecropin A
membranes
NMR spectroscopy
trimethyllysine
7 pag.- 6 fig.- 1 tab.
The 3D structures of six linear pentadecapeptides derived from the cecropin A–melittin antimicrobial peptide CA(1–7)M(2–9) [KWKLFKKIGAVLKVL-NH2] have been studied. These analogues are modified by ε-NH2 trimethylation of one or more lysine residues and showed variation in both antimicrobial and cytotoxic activities, depending on the number and position of modified lysines. Since it is expected that these peptides will display a strong conformational ordering when in contact with membranes, we have investigated their structure on the basis of the data extracted from NMR experiments performed in membrane-mimetic environments. We show that inclusion of Nε-trimethylated lysine residues induces a certain degree of structural flexibility, while preserving to a variable extent a largely α-helical structure. In addition, peptide orientation with respect to SDS micelles has been explored by detection of the intensity changes of peptide NMR signals upon addition of a paramagnetic probe (Mn2+ ions)
M.D.D. gratefully acknowledges support through a “Ramón y Cajal” contract from Ministerio de Ciencia e Innovación (MICINN) of Spain. J.J.B. thanks also MICINN for financial support (grant CTQ2009-08536). L.R. is supported by projects FIS 09/01928, RD 06/0021/0006 and EC HEALTH M-2007-223414. We also thank CESGA (Santiago de Compostela) for computer support
Peer reviewed
2011-09-19
artículo
http://purl.org/coar/resource_type/c_6501
Chembiochem 12(14):2177-2183(2011)
1439-4227
http://hdl.handle.net/10261/50854
10.1002/cbic.201100269
1439-7633
en
http://dx.doi.org/10.1002/cbic.201100269
none
Wiley-VCH