2024-03-19T13:07:44Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/487292018-08-07T10:35:54Zcom_10261_14com_10261_8col_10261_267
Larval organogenesis of Pagrus pagrus
Darias, M. J.
Ortiz-Delgado, Juan B.
Sarasquete, Carmen
Martínez-Rodríguez, Gonzalo
Yúfera, Manuel
Gut development
Pagrus pagrus
Red porgy
Histology
Histochemistry
Fish larvae
16 páginas, 8 figuras, 6 tablas.
Organogenesis of the red porgy (Pagrus pagrus L., 1758) was examined from hatching until 63 days post-hatching (dph) using histological and histochemical techniques. At hatching, the heart appeared as a tubular structure which progressively developed into four differentiated regions at 2 dph: bulbus arteriosus, atrium, ventricle and sinus venosus. First ventricle and atrium trabeculae were appreciated at 6 and 26 dph, respectively. Primordial gill arches were evident at 2 dph. Primordial filaments and first lamellae were observed at 6 and 15 dph, respectively. At mouth opening (3dph), larvae exhausted their yolk-sac reserves. The pancreatic zymogen granules appeared at 6 dph. Glycogen granules, proteins and neutral lipids (vacuoles in paraffin sections) were detected in the cytoplasm of the hepatocytes from 4-6 dph. Hepatic sinusoids could be observed from 9 dph. Pharyngeal and buccal teeth were observed at 9 and 15 dph, respectively. Oesophageal goblet cells appeared around 6 dph, containing neutral and acid mucosubstances. An incipient stomach could be distinguished at 2 dph. The first signs of gastric gland development were detected at 26 dph, increasing in number and size by 35-40 dph. Gastric glands were concentrated in the cardiac stomach region and presented a high content of protein rich in tyrosine, arginine and tryptophan. The intestinal mucous cells appeared at 15 dph and contained neutral and acid glycoconjugates, the carboxylated mucins being more abundant than the sulphated ones. Acidophilic supranuclear inclusions in the intestinal cells of the posterior intestine, related to pynocitosis of proteins, were observed at 4-6 dph.
This work was supported by the Comisión
Interministerial de Ciencia y Tecnología, Spain (CICYT Projects AGL2000-0697-C02-01 and AGL2003-03558). M.J. Darias was
supported by a FPI fellowship from MCYT, Spain (FP2000-5265). J.B. Ortiz-Delgado was supported by a Juan de la Cierva Postdoctoral
Contract from MCYT, Spain.
Peer reviewed
2012-04-23T12:14:02Z
2012-04-23T12:14:02Z
2007
artículo
http://purl.org/coar/resource_type/c_6501
Histology and Histopathology (Cellular and Molecular Biology ) 22(7): 753-768 (2007)
0213-3911
http://hdl.handle.net/10261/48729
1699-5848
en
http://www.hh.um.es/Abstracts/Vol_22/22_7/22_7_753.htm
open
Universidad de Murcia