2024-03-28T13:42:32Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2436642021-12-27T15:47:49Zcom_10261_75com_10261_6col_10261_328
Persulfidation of ATG18a regulates autophagy under ER stress in Arabidopsis
Aroca, Ángeles
Yruela Guerrero, Inmaculada
Gotor, Cecilia
Bassham, Diane C.
European Commission
Gobierno de Aragón
Agencia Estatal de Investigación (España)
Yruela Guerrero, Inmaculada [0000-0003-3608-4720]
ATG18a
ER stress
Autophagy
hydrogen sulfide
persulfidation.
2 .pdfs (Article -18 p.- 7 Figs.- ; Supp. Inf. -12 Pags.- 5 Figs.- 1 Tabl.- ). The definitive version is available at: https://www.pnas.org/
Hydrogen sulfide (H2S) is an endogenously generated gaseous signaling molecule, which recently has been implicated in autophagy regulation in both plants and mammals through persulfidation of specific targets. Persulfidation has been suggested as the molecular mechanism through which sulfide regulates autophagy in plant cells. ATG18a is a core autophagy component that is required for bulk autophagy and also for reticulophagy during endoplasmic reticulum (ER) stress. In this research, we revealed the role of sulfide in plant ER stress responses as a negative regulator of autophagy. We demonstrate that sulfide regulates ATG18a phospholipid-binding activity by reversible persulfidation at Cys103, and that this modification activates ATG18a binding capacity to specific phospholipids in a reversible manner. Our findings strongly suggest that persulfidation of ATG18a at C103 regulates autophagy under ER stress, and that the impairment of persulfidation affects both the number and size of autophagosomes.
This work was supported in part by the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie Grant Agreement No. 834120. We also acknowledge the Project US-1255781 European Regional Development Fund for funding support (to A.A.), Government of Aragón FEDER Grant E35_17R (to I.Y.), Agencia Estatal Investigación European Regional Development Fund Grant PID2019-109785GB-I00 (to C.G.), and NIH Grant 1R01GM120316-01A1 (to D.C.B.).
Peer reviewed
2021-06-16T08:15:01Z
2021-06-16T08:15:01Z
2021-05
artículo
http://purl.org/coar/resource_type/c_6501
Aroca A, Yruela I, Gotor C, Bassham DC. Persulfidation of ATG18a regulates autophagy under ER stress in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 118 (20): e2023604118 (2021)
0027-8424
http://hdl.handle.net/10261/243664
10.1073/pnas.2023604118
1091-6490
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100010067
33975948
en
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/834120
Postprint
https://doi.org/10.1073/pnas.2023604118
Sí
open
National Academy of Sciences (U.S.)