2024-03-28T23:32:46Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2259932022-10-04T08:29:55Zcom_10261_123com_10261_8col_10261_376
Gene expression during bacterivorous growth of a widespread marine heterotrophic flagellate
Massana, Ramon
Labarre, Aurélie
López-Escardó, David
Obiol, Aleix
Bucchini, François
Hackl, Thomas
Fischer, Matthias G.
Vandepoele, Klaas
Tikhonenkov, Denis V.
Keeling, Patrick J.
Ministerio de Economía y Competitividad (España)
Russian Foundation for Basic Research
European Commission
Agencia Estatal de Investigación (España)
14 pages, 6 figures, 2 tables, supplementary information https://doi.org/10.1038/s41396-020-00770-4
Phagocytosis is a fundamental process in marine ecosystems by which prey organisms are consumed and their biomass incorporated in food webs or remineralized. However, studies searching for the genes underlying this key ecological process in free-living phagocytizing protists are still scarce, in part due to the lack of appropriate ecological models. Our reanalysis of recent molecular datasets revealed that the cultured heterotrophic flagellate Cafeteria burkhardae is widespread in the global oceans, which prompted us to design a transcriptomics study with this species, grown with the cultured flavobacterium Dokdonia sp. We compared the gene expression between exponential and stationary phases, which were complemented with three starvation by dilution phases that appeared as intermediate states. We found distinct expression profiles in each condition and identified 2056 differentially expressed genes between exponential and stationary samples. Upregulated genes at the exponential phase were related to DNA duplication, transcription and translational machinery, protein remodeling, respiration and phagocytosis, whereas upregulated genes in the stationary phase were involved in signal transduction, cell adhesion, and lipid metabolism. We identified a few highly expressed phagocytosis genes, like peptidases and proton pumps, which could be used to target this ecologically relevant process in marine ecosystems
his research was supported by the Spanish Ministry of Economy and Competitiveness project ALLFLAGS (CTM2016-75083-R) to RM, by the Russian Foundation for Basic Research (grant No 18-504-51028) within the framework of Ministry of Education and Science of the Russian Federation project No АААА-А18-118012690098-5 to DVT, and by the European Union Marie Skłodowska-Curie Grant agreement H2020-MSCA-ITN-2015-675752 to AL and FB
With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI)
Peer reviewed
2021-01-05T07:57:30Z
2021-01-05T07:57:30Z
2021-01
artículo
http://purl.org/coar/resource_type/c_6501
ISME Journal - International Society for Microbial Ecology 15: 154-167 (2021)
1751-7362
CEX2019-000928-S
http://hdl.handle.net/10261/225993
10.1038/s41396-020-00770-4
1751-7370
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100002261
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100011033
32920602
en
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2016-75083-R
info:eu-repo/grantAgreement/EC/H2020/675752
Publisher's version
https://doi.org/10.1038/s41396-020-00770-4
Sí
open
International Society for Microbial Ecology