2024-03-29T14:29:19Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2184322020-10-07T01:14:10Zcom_10261_62com_10261_6col_10261_188
Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis
Martínez, María Teresa
Viéitez Madriñán, Francisco Javier
Solla, Alejandro
Tapias, Raúl
Ramírez-Martín, Noelia
Corredoira, Elena
Xunta de Galicia
Junta de Andalucía
European Commission
Axillary shoot proliferation
Clonal propagation
Micropropagation
Phytophthora cinnamomi
Quercus ilex
Silver thiosulphate
Somatic embryogenesis
Disease-tolerant plants
© 2020 by the authors.
Holm oak (Quercus ilex) is one of the most widely distributed tree species in the Mediterranean basin. High mortality rates have been observed in holm oak populations in the southwest of the Iberian Peninsula as a result of oak decline syndrome. Selection and propagation of genotypes tolerant to this syndrome could aid the restoration of affected areas. In this article, we report micropropagation and conservation procedures based on axillary budding and somatic embryogenesis (SE) of holm oak plants, selected for their tolerance to Phytophthora cinnamomi—the main biotic factor responsible for oak decline. Forced shoots were obtained from potted plants of eight different genotypes, and used as stock material to establish in vitro shoot proliferation cultures. Reliable shoot proliferation was obtained in seven out the eight genotypes established in vitro, whereas multiplication rates were genotype-dependent. The highest rooting rates were obtained by culturing shoots for 24 h or 48 h on rooting induction medium containing 25 mg L−1 indole-3-butyric acid, followed by transfer to medium supplemented with 20 µM silver thiosulphate. Axillary shoot cultures can be successful conserved by cold storage for 12 months at 4 °C under dim lighting. Shoot tips, excised from axillary shoot cultures established from tolerant plants, were used as initial explants to induce SE. Somatic embryos and/or nodular embryogenic structures were obtained on induction medium with or without indole-acetic acid 4 mg L−1, in two out the three genotypes evaluated, and induction rates ranged between 2 and 4%. Plantlet recovery was 45% after two months cold stratification of somatic embryos and eight weeks of culture on germination medium. Vegetative propagation of P. cinnamomi-tolerant Q. ilex trees is a valuable milestone towards the restoration of disease-affected areas.
This research was partly funded by Xunta de Galicia (Spain) through the project Contrato Programa 2018–2019. Selection of UHu genotypes was funded by the Andalusian Regional Ministry of Science, Innovation and Busi-ness (project P07-RNM-03108), the European Social Fund and European Regional Development Fund.
Peer reviewed
2020-08-21T13:55:03Z
2020-08-21T13:55:03Z
2020
2020-08-21T13:55:04Z
artículo
http://purl.org/coar/resource_type/c_6501
Forests 11(8): 841 (2020)
http://hdl.handle.net/10261/218432
10.3390/f11080841
1999-4907
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100010801
http://dx.doi.org/10.13039/501100011011
en
Publisher’s version
https://doi.org/10.3390/f11080841
Sí
open
Multidisciplinary Digital Publishing Institute