2024-03-28T15:45:34Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2164772021-12-27T16:48:08Zcom_10261_86com_10261_1col_10261_339
The SlCBL10 calcineurin B-like protein ensures plant growth under salt stress by regulating Na+ and Ca2+ homeostasis
Egea, Isabel
Pineda, Benito
Ortíz-Atienza, Ana
Plasencia, Félix A.
Drevensek, Stéphanie
García-Sogo, Begoña
Yuste-Lisbona, Fernando J.
Barrero-Gil, J.
Atarés Huerta, Alejandro
Flores, Francisco B.
Barneche, Fredy
Angosto Trillo, Trinidad
Capel, Carmen
Salinas, Julio
Vriezen, Wim
Esch, Elizabeth
Bowler, Chris
Bolarín, María C.
Moreno Ferrero, Vicente
Lozano, Rafael
PLANT-KBBE
Ministerio de Economía y Competitividad (España)
Agence Nationale de la Recherche (France)
Egea, Isabel [0000-0002-1248-0297]
Pineda, Benito [0000-0001-9176-701X]
Ortíz-Atienza, Ana [0000-0003-1750-4785]
Plasencia, Félix A. [0000-0001-9353-990X]
Drevensek, Stéphanie [0000-0002-2356-7021]
Yuste-Lisbona, Fernando J. [0000-0001-9222-7293]
Barrero-Gil, J. [0000-0003-0878-7576]
Atarés Huerta, Alejandro [0000-0003-1728-5471]
Flores, Francisco B. [0000-0002-9883-9458]
Barneche, Fredy [0000-0002-7014-7097]
Angosto Trillo, Trinidad [0000-0003-0294-9255]
Salinas, Julio [0000-0003-2020-0950]
Vriezen, Wim [0000-0001-8189-201X]
Esch, Elizabeth [0000-0002-8760-3247]
Bowler, Chris [0000-0003-3835-6187]
Bolarín, María C. [0000-0002-2662-4917]
Moreno Ferrero, Vicente [0000-0002-0345-7300]
Lozano, Rafael [0000-0001-5458-2075]
Calcium sensor cbl10
Vacuolar h+-atpase
Arabidopsis thaliana
Salinity tolerance
Ion homeostasis
Enhancer trap
SOS pathway
Tomato
Transporters
Expression
18 p.-9 fig.
Characterization of a new tomato (Solanum lycopersicum) T-DNA mutant allowed for the isolation of the CALCINEURIN B-LIKE PROTEIN 10 (SlCBL10) gene whose lack of function was responsible for the severe alterations observed in the shoot apex and reproductive organs under salinity conditions. Physiological studies proved that SlCBL10 gene is required to maintain a proper low Na+/Ca2+ ratio in growing tissues allowing tomato growth under salt stress. Expression analysis of the main responsible genes for Na+ compartmentalization (i.e. Na+/H+ EXCHANGERs, SALT OVERLY SENSITIVE, HIGH-AFFINITY K+ TRANSPORTER 1; 2, H+-pyrophosphatase AVP1 [SlAVP1] and V-ATPase [SlVHA-A1]) supported a reduced capacity to accumulate Na+ in Slcbl10 mutant leaves, which resulted in a lower uploading of Na+ from xylem, allowing the toxic ion to reach apex and flowers. Likewise, the tomato CATION EXCHANGER 1 and TWO-PORE CHANNEL 1 (SlTPC1), key genes for Ca2+ fluxes to the vacuole, showed abnormal expression in Slcbl10 plants indicating an impaired Ca2+ release from vacuole. Additionally, complementation assay revealed that SlCBL10 is a true ortholog of the Arabidopsis (Arabidopsis thaliana) CBL10 gene, supporting that the essential function of CBL10 is conserved in Arabidopsis and tomato. Together, the findings obtained in this study provide new insights into the function of SlCBL10 in salt stress tolerance. Thus, it is proposed that SlCBL10 mediates salt tolerance by regulating Na+ and Ca2+ fluxes in the vacuole, cooperating with the vacuolar cation channel SlTPC1 and the two vacuolar H+-pumps, SlAVP1 and SlVHA-A1, which in turn are revealed as potential targets of SlCBL10.
Peer reviewed
2020-07-10T13:47:11Z
2020-07-10T13:47:11Z
2018-02
artículo
http://purl.org/coar/resource_type/c_6501
Plant Physiology 176 (2) 1676-1693 (2018)
0032-0889
http://hdl.handle.net/10261/216477
10.1104/pp.17.01605
1532-2548
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100001665
29229696
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64991-C3-1-R/2-R/3-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-79187-R
https://doi.org/10.1104/pp.17.01605
Sí
none
American Society of Plant Biologists