2024-03-28T10:48:28Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2159562021-12-27T16:43:02Zcom_10261_108com_10261_8col_10261_361
Reduction in oviposition of poultry red mite (Dermanyssus gallinae) in hens vaccinated with recombinant akirin
Lima-Barbero, José Francisco
Contreras, Marinela
Bartley, Kathryn
Price, Daniel R.G.
Nunn, Francesca
Sánchez Sánchez, Marta
Prado, Eduardo
Höfle, Ursula
Villar, Margarita
Nisbet, Alasdair J.
Fuente, José de la
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Universidad de Castilla La Mancha
Lima-Barbero, José Francisco [0000-0002-2694-0215]
Contreras, Marinela [0000-0002-1505-5624]
Höfle, Ursula [0000-0002-6868-079X]
Villar, Margarita [0000-0003-4172-9079]
Fuente, José de la [0000-0001-7383-9649]
Dermanyssus
Akirin
Vaccine
Poultry red mite
Tick
Subolesin
The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite of birds with worldwide distribution that causes economic losses in the egg-production sector of the poultry industry. Traditional control methods, mainly based on acaricides, have been only partially successful, and new vaccine-based interventions are required for the control of PRM. Vaccination with insect Akirin (AKR) and its homolog in ticks, Subolesin (SUB), have shown protective efficacy for the control of ectoparasite infestations and pathogen infection/transmission. The aim of this study was the identification of the akr gene from D. gallinae (Deg-akr), the production of the recombinant Deg-AKR protein, and evaluation of its efficacy as a vaccine candidate for the control of PRM. The anti-Deg-AKR serum IgY antibodies in hen sera and egg yolk were higher in vaccinated than control animals throughout the experiment. The results demonstrated the efficacy of the vaccination with Deg-AKR for the control of PRM by reducing mite oviposition by 42% following feeding on vaccinated hens. A negative correlation between the levels of serum anti-Deg-AKR IgY and mite oviposition was obtained. These results support Deg-AKR as a candidate protective antigen for the control of PRM population growth.
This work was partially supported by COST Action FA 1404 (COREMI) (https://www.coremi.eu/home.html). JFLB was supported by Doctorado Industrial contract DI-14-06917 from Ministerio de Ciencia, Innovación y Universidades (Spain) and Sabiotec S.A. MV was supported by the Research Plan of the University of Castilla-La Mancha (Spain).
Peer reviewed
2020-07-03T09:49:47Z
2020-07-03T09:49:47Z
2019
artículo
http://purl.org/coar/resource_type/c_6501
Vaccines 7(3): 121 (2019)
http://hdl.handle.net/10261/215956
10.3390/vaccines7030121
2076-393X
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100007480
31546944
en
Publisher's version
https://doi.org/10.3390/vaccines7030121
Sí
open
Multidisciplinary Digital Publishing Institute