2024-03-29T13:09:56Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2145642021-12-27T15:50:09Zcom_10261_25com_10261_1col_10261_278
NKG2H-expressing T cells negatively regulate immune responses
Dukovska, Daniela
Fernández-Soto, Daniel
Valés-Gómez, Mar
Reyburn, H. T.
Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Valés Gómez, Mar [0000-0001-7424-3206]
nKg2h, T cells, negative regulation, DaP12, cell–cell interaction
The biology and function of NKG2H receptor, unlike the better characterized members of the NKG2 family NKG2A, NKG2C, and NKG2D, remains largely unclear. Here, we show that NKG2H is able to associate with the signaling adapter molecules DAP12 and DAP10 suggesting that this receptor can signal for cell activation. Using a recently described NKG2H-specific monoclonal antibody (mAb), we have characterized the expression and function of lymphocytes that express this receptor. NKG2H is expressed at the cell surface of a small percentage of peripheral blood mononuclear cell (PBMC) and is found more frequently on T cells, rather than NK cells. Moreover, although NKG2H is likely to trigger activation, co-cross-linking of this receptor with an NKG2H-specific mAb led to decreased T cell activation and proliferation in polyclonal PBMC cultures stimulated by anti-CD3 mAbs. This negative regulatory activity was seen only after cross-linking with NKG2H, but not NKG2A- or NKG2C-specific monoclonal antibodies. The mechanism underlying this negative effect is as yet unclear, but did not depend on the release of soluble factors or recognition of MHC class I molecules. These observations raise the intriguing possibility that NKG2H may be a novel marker for T cells able to negatively regulate T cell responses.
Work was supported by grants from the Fondo de Investigación Sanitaria (PI11/00298 and PI08/1701), MINECO (SAF2012-32293, SAF2015-69169-R, and SAF2014-58752-R), and the Regional Government of Madrid (grant S2010/BMD2326). DD was funded by an International predoctoral fellowship from La Caixa foundation.
2020-06-16T13:50:05Z
2020-06-16T13:50:05Z
2018
2020-06-16T13:50:05Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.3389/fimmu.2018.00390
e-issn: 1664-3224
pmid: 29545803
Frontiers in Immunology 9: 390 (2018)
http://hdl.handle.net/10261/214564
10.3389/fimmu.2018.00390
http://dx.doi.org/10.13039/100012818
http://dx.doi.org/10.13039/501100003329
29545803
Publisher's version
http://dx.doi.org/10.3389/fimmu.2018.00390
Sí
open
Frontiers Media