2024-03-28T07:56:27Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1999032021-09-16T05:57:16Zcom_10261_123com_10261_8col_10261_376
Immune genes, IL1β and Casp9, show sexual dimorphic methylation patterns in zebrafish gonads
Caballero-Huertas, Marta
Moraleda, J.
Joly, Sílvia
Ribas, Laia
Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Immune
Epimarkers
Methylation
Gene expression
Gonads
8 pages, 6 figures, supplementary data https://doi.org/10.1016/j.fsi.2019.12.013
There is crosstalk between the immune and reproductive systems in which sexual dimorphism is a common pattern in vertebrates. In recent years, epigenetics has emerged as a way to study the molecular mechanisms involved in gonadal development, those responsible for integrating environmental information that contribute to assigning a specific sexual phenotype (either an ovary or a testis). The knowledge of epigenetic mechanisms in certain molecular processes allows the development of epigenetic markers. In fish gonads, the existence of reproduction-immune system interactions is known, although the epigenetic mechanisms involved are far from clear. Here, we used the zebrafish (Danio rerio) as a model to study the DNA methylation patterns in gonads of two well-known innate immune genes: IL1β and Casp9. DNA methylation levels were studied by a candidate gene approach at single nucleotide resolution and gene expression analyses were also carried out. Results showed that there was clear sexual dimorphism in the DNA methylation levels of the two immune genes studied, being significantly higher in the testes when compared to the ovaries. In summary, and although further research is needed, this paper presents sexual dimorphic methylation patterns of two immune-related genes, thus sex-biased differences in methylation profiles should considered when analyzing immune responses in fish. Data showed here can help to develop epimarkers with forthcoming applications in livestock and fish farming production, for example, in immune fish diseases or sexual control programs as epigenetic molecular tools to predict environmental pressure in the gonads
This study was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) grant AGL2015-73864-JIN “Ambisex” to LR. LR and JM were supported by “Ambisex” contracts
With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI)
2020-02-06T13:41:29Z
2020-02-06T13:41:29Z
2020-02
2020-02-06T13:41:29Z
artículo
http://purl.org/coar/resource_type/c_6501
issn: 1050-4648
e-issn: 1095-9947
Fish and Shellfish Immunology 97: 648-655 (2020)
CEX2019-000928-S
http://hdl.handle.net/10261/199903
10.1016/j.fsi.2019.12.013
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100011033
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-73864-JIN
https://doi.org/10.1016/j.fsi.2019.12.013
Sí
open
Academic Press