2024-03-29T09:27:34Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1889602022-06-01T12:30:37Zcom_10261_105com_10261_1com_10261_86col_10261_358col_10261_339
Role of Resveratrol and Selenium on Oxidative Stress and Expression of Antioxidant and Anti-Aging Genes in Immortalized Lymphocytes from Alzheimer’s Disease Patients
Cosín-Tomás, Marta
Senserrich, Júlia
Arumí-Planas, Marta
Alquézar, Carolina
Pallàs, Mercè
Martín-Requero, Ángeles
Suñol, Cristina
Kaliman, Perla
Sanfeliu, Coral
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Generalitat de Catalunya
Alzheimer’s disease
Immortalized B lymphoblastoid cell lines
Resveratrol
Selenium
Oxidative stress
Aging
Gene expression
Oxidative damage is involved in the pathophysiology of age-related ailments, including Alzheimer’s disease (AD). Studies have shown that the brain tissue and also lymphocytes from AD patients present increased oxidative stress compared to healthy controls (HCs). Here, we use lymphoblastoid cell lines (LCLs) from AD patients and HCs to investigate the role of resveratrol (RV) and selenium (Se) in the reduction of reactive oxygen species (ROS) generated after an oxidative injury. We also studied whether these compounds elicited expression changes in genes involved in the antioxidant cell response and other aging-related mechanisms. AD LCLs showed higher ROS levels than those from HCs in response to H2O2 and FeSO4 oxidative insults. RV triggered a protective response against ROS under control and oxidizing conditions, whereas Se exerted antioxidant effects only in AD LCLs under oxidizing conditions. RV increased the expression of genes encoding known antioxidants (catalase, copper chaperone for superoxide dismutase 1, glutathione S-transferase zeta 1) and anti-aging factors (sirtuin 1 and sirtuin 3) in both AD and HC LCLs. Our findings support RV as a candidate for inducing resilience and protection against AD, and reinforce the value of LCLs as a feasible peripheral cell model for understanding the protective mechanisms of nutraceuticals against oxidative stress in aging and AD.
This research was funded by Spanish MINECO and FEDER, grant number SAF2016-75508; Catalan Autonomous Government AGAUR, grant number 2017-SGR-106; and the CERCA Programme/Generalitat de Catalunya.
Peer reviewed
2019-08-23T07:05:18Z
2019-08-23T07:05:18Z
2019-07-31
2019-08-23T07:05:18Z
artículo
http://purl.org/coar/resource_type/c_6501
Nutrients 11(8): 1764 (2019)
http://hdl.handle.net/10261/188960
10.3390/nu11081764
2072-6643
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100002809
http://dx.doi.org/10.13039/501100003329
31370365
en
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-75508-R
Publisher's version
https://doi.org/10.3390/nu11081764
Sí
open
Multidisciplinary Digital Publishing Institute