2024-03-28T14:08:44Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1790312020-07-02T11:37:21Zcom_10261_103com_10261_1col_10261_356
Regulation of xylem fibers differentiation by gibberellins through DELLA-KNAT1 interaction
Felipo-Benavent, Amelia
Úrbez, Cristina
Blanco-Touriñán, Noel
Serrano Mislata, Antonio
Baumberger, Nicolas
Achard, Patrick
Agustí, Javier
Blázquez, Miguel Ángel
Alabadí, David
Ministerio de Economía y Competitividad (España)
European Commission
Hormones
Plant vasculature
Secondary cell wall
Lignin
The thickening of plant organs is supported by secondary growth, a process by which new vascular tissues (xylem and phloem) are produced. Xylem is composed of several cell types, including xylary fibers, parenchyma and vessel elements. In Arabidopsis, it has been shown that fibers are promoted by the class-I KNOX gene KNAT1 and the plant hormones gibberellins, and repressed by a small set of receptor-like kinases, but we lack a mechanistic framework to integrate their relative contributions. Here we show that DELLAs, negative elements of the gibberellin signaling pathway, physically interact with KNAT1 and impair its binding to KNAT1 binding sites. Our analysis also indicates that at least 37% of the transcriptome mobilized by KNAT1 is potentially dependent on this interaction, and includes genes involved in secondary cell wall modifications and phenylpropanoid biosynthesis. Moreover, the promotion by constitutive overexpression of KNAT1 of fiber formation and the expression of genes required for fiber differentiation were still reverted by DELLA accumulation, in agreement with post-translational regulation of KNAT1 by DELLA proteins. These results suggest that gibberellins enhance fiber development by promoting KNAT1 activity.
This work was funded by the Spanish Agencia Estatal de Investigación (BFU2016-80621-P, BIO2016-71933-P and BIO2016-79147-R to MAB, DA and JA respectively). A.F-B. and N.B-T. were recipients of a MINECO FPI Fellowships (BES-2011-045689 and BES-2014-068868, respectively, and A.S-M and M.A.B. acknowledge funding of the European Union (H2020-MSCA-IF-2016-746396).
Peer reviewed
2019-04-01T11:13:24Z
2019-04-01T11:13:24Z
2018-11-26
artículo
http://purl.org/coar/resource_type/c_6501
Development 145: dev164962 (2018)
0950-1991
http://hdl.handle.net/10261/179031
10.1242/dev.164962
1477-9129
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
30389856
en
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info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-80621-P
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-71933-P
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-79147-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2013-43184-P
info:eu-repo/grantAgreement/EC/H2020/746396
Postprint
https://doi.org/10.1242/dev.164962
Sí
open
Company of Biologists