2024-03-28T09:29:52Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/174252020-07-03T12:48:41Zcom_10261_41com_10261_1com_10261_5062com_10261_5col_10261_294col_10261_5064
Integrated procedure for determination of endocrine-disrupting activity in surface waters and sediments by use of the biological technique recombinant yeast assay and chemical analysis by LC-ESI-MS
Céspedes, Raquel
Petrovic, Mira
Raldúa, Demetrio
Saura, Úrsula
Piña, Benjamín
Lacorte Bruguera, Silvia
Viana, Paula
Barceló, Damià
Endocrine-disrupting compounds
Estrogenicity
Recombinant yeast assay
Liquid chromatography-mass spectrometry
12 pages, 5 figures, 4 tables.-- PMID: 14658021 [PubMed].-- Available online Dec 5, 2003.
An integrated procedure using mass spectrometry and molecular biology for determination of estrogenicity in natural waters and sediments is reported. Solid-phase extraction (SPE) and pressurized-liquid extraction (PLE), respectively, were used for isolation of endocrine-disrupting compounds (EDC) from surface waters and sediments, followed by liquid chromatography–mass spectrometry using an electrospray interface (LC–ESI-MS). Twenty seven EDC were determined: non-ionic surfactants (nonylphenol ethoxylate), alkylphenols (e.g. nonylphenol and octylphenol), bisphenol A, phthalates, and natural and synthetic steroid sex hormones. Limits of detection varied from 0.02 to 0.22 μg L–1 and from 1 to 10 μg kg–1 in water and sediments, respectively. Recoveries ranged from 65 to 125% and 73 to 97% for waters and sediments, respectively. In addition to LC–ESI-MS determination, extracts obtained by SPE and PLE were analyzed by the recombinant yeast assay (RYA) to assess total estrogenic activity. This bioassay detects natural estrogens and xenoestrogens, producing a quantitative measurement of EDC irrespective of the identity of the chemical responsible for the activity. As a novelty, a relative estrogenicity factor was determined for 19 analytes with EC50 values ranging from 10–10 to 10–9 mol L–1 for synthetic estrogens, from 10–7 to 10–5 mol L–1 for alkylphenol derivatives, and from 10–5 to 10–4 mol L–1 for phthalates and benzothiazoles. By use of this integrated chemical–ecotoxicological approach good correlation was usually established between chemical composition and estrogenic effects for surface water and sediment samples from Portugal. Estrogenic activity observed was mainly attributed to the presence of nonylphenolic compounds (with concentrations of NP ranging from 0.1 up to 44 μg L–1 in waters and up to 1172 μg kg–1 in sediments), and to the sporadic presence of estrogens, detected at ng L–1 levels.
This work was supported by the Portuguese
Instituto do Ambiente (Ministerio das Cidades, Ordenamiento Territorio e Ambiente) and by Ministerio de Ciencia y Tecnología (Spain) (BMC2001–0246).
Peer reviewed
2009-10-05T08:05:47Z
2009-10-05T08:05:47Z
2004-02-04
artículo
http://purl.org/coar/resource_type/c_6501
Analytical and Bioanalytical Chemistry 378(3): 697-708 (2004)
1618-2642
http://hdl.handle.net/10261/17425
10.1007/s00216-003-2303-5
1618-2650
en
http://dx.doi.org/10.1007/s00216-003-2303-5
none
22195 bytes
application/pdf
Springer