2024-03-28T20:03:41Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1737642019-01-05T01:55:30Zcom_10261_14com_10261_8col_10261_267
Molecular characterization and transcriptional regulation by GH and GnRH of insulin-like growth factors I and II in white seabream (Diplodus sargus)
Pérez, Laura
Ortiz-Delgado, Juan B.
Manchado, Manuel
European Commission
European Commission
Gene expression
Growth hormone
IGF-I
IGF-II
Insulin-like growth factors
Diplodus sargus
Insulin-like growth factors (IGF) I and II are key regulators of development, growth and reproduction in fish. In the present study we have cloned and characterized the cDNA and genomic sequences of IGF-I and IGF-II in the white seabream (Diplodus sargus). The igf1 and igf2 genes were encoded putatively by five and four exons, respectively. Moreover, the 5′-flanking upstream region of the igf1 gene contained highly conserved regulatory elements including HNF-1α, HNF-3β, CCAAT/enhancer binding protein (C/EBP) and the TATA box. The full-length cDNAs were 1225 and 1666 nucleotides long for igf1 and igf2, respectively. Sequence analysis identified the A–E domains as well as three spliced forms involving the E domain in exons 3–5. ORF identities were higher than 83% with respect to other fish orthologs. Expression analysis demonstrated that igf1 and its spliced forms were mostly expressed in liver, whereas the igf2 was expressed ubiquitously not detecting significant differences among the ten tissues analyzed. Hormonal treatments using the porcine GH demonstrated a sharply increase of both igf1 and igf2 mRNA levels in liver and gills at 30 min and 1 h after injection. In the gonads, igf1 mRNA levels increased steadily with testis and ovary maturation. In contrast, igf2 transcript amounts were higher in immature stages (S1–S2). Hormonal treatments using GH and GnRH demonstrated that igf1 and igf2 expression were upregulated in the gonads. Overall, these data demonstrate that IGF-I and IGF-II are locally expressed in several tissues and regulated by key hormones of the somatotropic and gonadotropic axes.
This study has been funded by a FEP (European Union) and Dirección General de Pesca de la Consejeria de Agricultura y Pesca, Junta de Andalucía.
Peer reviewed
2019-01-04T12:29:41Z
2019-01-04T12:29:41Z
2016-03-10
artículo
http://purl.org/coar/resource_type/c_6501
Gene 578(2): 251-262 (2016)
0378-1119
http://hdl.handle.net/10261/173764
10.1016/j.gene.2015.12.030
http://dx.doi.org/10.13039/501100000780
en
https://doi.org/10.1016/j.gene.2015.12.030
Sí
none
Elsevier