2024-03-29T09:50:57Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1718752019-10-04T07:54:27Zcom_10261_123com_10261_8com_10261_5062com_10261_5col_10261_502col_10261_5070
UHPLC-HRMS determination of palytoxin and ovatoxins from Ostreopsis cf. ovata, a marine microalgae blooming in a Northwestern Mediterranean beach
Moyano, Encarnación
Alechaga, Élida
Caixach Gamisans, Josep
Lacorte Bruguera, Silvia
Palacios, Óscar
Farràs, Jaume
Vila, Magda
Viure Feliu, Laia
Berdalet, Elisa
Ministerio de Economía y Competitividad (España)
21st International Mass Spectrometry Conference (IMSC 2016), 20-27 August 2016, Toronto, Canada
Introduction: Blooms of Ostreopsis cf. ovata (palytoxin/ovatoxins-producing microalgae) are spreading to coastal areas of temperate latitudes and constitute a health and environmental emergent problem. The risks posed by these events will only be determined if toxin patterns are identified and quantified. Nevertheless, the mass spectrometry characterization of palytoxin/ovatoxins is already a challenge.
Methods: Ultra-high performance reversed phase liquid chromatography coupled to electrospray-high resolution mass spectrometry (UHPLC- HRMS) was applied for the reliable identification and determination of palytoxin/ovatoxins. Extraction, UHPLC and HRMS protocols were optimized to quantify the different toxins along an Ostreopsis cf. ovata bloom in Sant Andreu de Llavaneres beach (Barcelona, Spain).
Results: Ovatoxins are complex macromolecules closely related to Palytoxin, whose mass spectrometry characterization is already a challenge. The complex electrospray mass spectra show multiply charged ions, mixed cationized species and adducts, thus requiring efficient chromatographic separations of potential interfering species and the careful selection of diagnostic ions for their analysis. The relative intensity variation of cationized species from sample-to-sample and from standards hinders quantitative analysis, thus entailing the use of integrated signals from selected non-interfering ions. Microalgae samples from a Mediterranean beach were analyzed during an Ostreopsis bloom in 2015. Putative palytoxin and ovatoxins were detected, being ovatoxin-a the dominant component. The highest concentration per cell almost coincided with maximum Ostreopsis abundances.
Novel Aspect: The improved UHPLC-HRMS method evidences the presence of putative palytoxin and ovatoxins in the Ostreopsis bloom in a NW Mediterranean beach.
This work was financed by the Spanish Ministry of Economy and Competitiveness (MINECO) under the projects CTQ2015-63968-C2-1-P and OSTREORISK CTM2014-53818-R
Peer Reviewed
2018-11-05T13:42:45Z
2018-11-05T13:42:45Z
2016-08-20
2018-11-05T13:42:46Z
póster de congreso
http://purl.org/coar/resource_type/c_6670
21st International Mass Spectrometry Conference (2016)
http://hdl.handle.net/10261/171875
http://dx.doi.org/10.13039/501100003329
#PLACEHOLDER_PARENT_METADATA_VALUE#
MINECO/ICTI2013-2016/CTM2014-53818-R
Sí
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