2024-03-29T09:01:28Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1703962021-12-28T16:14:23Zcom_10261_59com_10261_6col_10261_312
The Pseudomonas putida T6SS is a plant warden against phytopathogens
Bernal Guzmán, Patricia
Allsopp, Luke P.
Filloux, Alain
Llamas Lorente, María A.
Biotechnology and Biological Sciences Research Council (UK)
Ministerio de Economía y Competitividad (España)
Junta de Andalucía
European Commission
Bacterial type VI secretion systems (T6SSs) are molecular weapons designed to deliver toxic effectors into prey cells. These nanomachines have an important role in inter-bacterial competition and provide advantages to T6SS active strains in polymicrobial environments. Here we analyze the genome of the biocontrol agent Pseudomonas putida KT2440 and identify three T6SS gene clusters (K1-, K2- and K3-T6SS). Besides, 10 T6SS effector-immunity pairs were found, including putative nucleases and pore-forming colicins. We show that the K1-T6SS is a potent antibacterial device, which secretes a toxic Rhs-type effector Tke2. Remarkably, P. putida eradicates a broad range of bacteria in a K1-T6SS-dependent manner, including resilient phytopathogens, which demonstrates that the T6SS is instrumental to empower P. putida to fight against competitors. Furthermore, we observed a drastically reduced necrosis on the leaves of Nicotiana benthamiana during co-infection with P. putida and Xanthomonas campestris. Such protection is dependent on the activity of the P. putida T6SS. Many routes have been explored to develop biocontrol agents capable of manipulating the microbial composition of the rhizosphere and phyllosphere. Here we unveil a novel mechanism for plant biocontrol, which needs to be considered for the selection of plant wardens whose mission is to prevent phytopathogen infections.
We thank Milagros Lopez (IVIA, Spain) for providing X. campestris and P. carotovorum, Martin Buck (Imperial College London, UK) for P. syringae and Ehr Min Lai (Academia Sinica, Taiwan) for A. tumefaciens and the pRL662-gpf. We thank Tom Wood for kindly providing the T6SS scheme shown in Figure 1a. PB is supported by the Spanish Ministry of Economy through Juan de la Cierva grant (JCI-2010-06615), by the Andalusian Knowledge Agency through a Talent Hub grant (TAHUB-010) and by an EMBO short-term fellowship. MAL is supported by the Spanish Ministry of Economy through a Ramon&Cajal grant (RYC2011-08874). AF is supported by a BBSRC grant (BB/N002539/1). LA is supported by a BBSRC grant (BB/N002539/1) and a Marie curie Fellowship (PIIF-GA-2012-328261).
Peer Reviewed
2018-10-01T10:45:15Z
2018-10-01T10:45:15Z
2017
2018-10-01T10:45:15Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1038/ismej.2016.169
issn: 1751-7370
ISME Journal 11: 972- 987 (2017)
http://hdl.handle.net/10261/170396
10.1038/ismej.2016.169
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100000268
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100011011
28045455
Publisher's version
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open
Nature Publishing Group