2024-03-29T00:02:57Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1632482020-12-10T17:15:42Zcom_10261_128com_10261_1col_10261_381
dachshund potentiates Hedgehog signaling during Drosophila retinogenesis
Bras-Pereira, Catarina
Potier, Delphine
Jacobs, Jelle
Aerts, Stein
Casares, Fernando
Janody, Florence
Fundação para a Ciência e a Tecnologia (Portugal)
Research Foundation - Flanders
Ministerio de Economía y Competitividad (España)
Proper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which coordinates cell fate determination, cell cycle and tissue morphogenesis. Here we show that during Drosophila retinogenesis, the retinal determination gene dachshund (dac) is not only a target of the Hh signaling pathway, but is also a modulator of its activity. Using developmental genetics techniques, we demonstrate that dac enhances Hh signaling by promoting the accumulation of the Gli transcription factor Cubitus interruptus (Ci) parallel to or downstream of fused. In the absence of dac, all Hh-mediated events associated to the morphogenetic furrow are delayed. One of the consequences is that, posterior to the furrow, dac- cells cannot activate a Roadkill-Cullin3 negative feedback loop that attenuates Hh signaling and which is necessary for retinal cells to continue normal differentiation. Therefore, dac is part of an essential positive feedback loop in the Hh pathway, guaranteeing the speed and the accuracy of Drosophila retinogenesis.
This work was supported by grants from Fundação para a Ciência e Tecnologia (PTDC/BIA-BCM/71674/2006) to FJ; from BFU2012-34324 and BFU2015-66040 (MINECO, Spain) to FC and The Research Foundation—Flanders (FWO, www.fwo.be) (grants G.0640.13 and G.0791.14) to SA. CBP was the recipient of fellowship from Fundação para a Ciência e Tecnologia (grant SFRH/BPD/46983/2008) and FJ is the recipient of IF/01031/2012 from Fundação para a Ciência e Tecnologia. JJ is the recipient of a FWO PhD Fellowship.
Peer Reviewed
2018-04-06T10:24:54Z
2018-04-06T10:24:54Z
2016
2018-04-06T10:24:54Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1371/journal.pgen.1006204
e-issn: 1553-7404
issn: 1553-7390
PLoS Genetics 12(7): e1006204 (2016)
http://hdl.handle.net/10261/163248
10.1371/journal.pgen.1006204
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100001871
http://dx.doi.org/10.13039/501100003130
27442438
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-66040-P
Publisher's version
https://doi.org/10.1371/journal.pgen.1006204
Sí
open
Public Library of Science