2024-03-30T04:20:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1628802021-08-24T10:47:55Zcom_10261_72com_10261_6col_10261_325
Interfamilial recombination between viruses led to acquisition of a novel translation enhancing RNA element that extends viral host range
Miras, Manuel
Sempere, Raquel N.
Kraft, Jelena J.
Miller, W. Allen
Aranda, Miguel A.
Truniger, Verónica
3′-UTR cap-independent translation enhancer (3′-CITE)
Cap-independent translation
Cucurbit aphid borne yellows virus (CABYV)
Eukaryotic translation initiation factor 4E (eIF4E)
Melon necrotic spot virus (MNSV)
Recessive resistance
Recombination
Resistance breaking
Many plant viruses depend on functional RNA elements, called 3′-UTR cap-independent
translation enhancers (3′-CITEs), for translation of their RNAs. In this manuscript we provide
direct proof for the existing hypothesis that 3′-CITEs are modular and transferable by recombination
in nature, and that this is associated with an advantage for the created virus.
By characterizing a newly identified Melon necrotic spot virus (MNSV; Tombusviridae) isolate,
which is able to overcome eukaryotic translation initiation factor 4E (eIF4E)-mediated
resistance, we found that it contains a 55 nucleotide insertion in its 3′-UTR. We provide strong
evidence that this insertion was acquired by interfamilial recombination with the 3′-UTR of an
Asiatic Cucurbit aphid-borne yellows virus (CABYV; Luteoviridae).
By constructing chimeric viruses, we showed that this recombined sequence is responsible
for resistance breaking. Analysis of the translational efficiency of reporter constructs showed
that this sequence functions as a novel 3′-CITE in both resistant and susceptible plants, being
essential for translation control in resistant plants.
In conclusion, we showed that a recombination event between two clearly identified viruses
from different families led to the transfer of exactly the sequence corresponding to a functional
RNA element, giving rise to a new isolate with the capacity to infect an otherwise nonsusceptible
host.
Peer reviewed
2018-03-26T09:53:14Z
2018-03-26T09:53:14Z
2014
artículo
http://purl.org/coar/resource_type/c_6501
New Phytologist 202 (1), 233-246 (2014)
http://hdl.handle.net/10261/162880
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