2024-03-19T05:59:26Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1584272021-12-27T15:33:00Zcom_10261_74com_10261_6col_10261_327
Large-Scale Transcriptome Analysis in Faba Bean (Vicia faba L.) under Ascochyta fabae Infection
Ocaña-Moral, Sara
Seoane, Pedro
Bautista, Rocío
Palomino, Carmen
Claros, Gonzalo M.
Torres, Ana M.
Madrid, Eva
European Commission
Junta de Andalucía
Faba bean is an important food crop worldwide. However, progress in faba bean genomics lags far behind that of model systems due to limited availability of genetic and genomic information. Using the Illumina platform the faba bean transcriptome from leaves of two lines (29H and Vf136) subjected to Ascochyta fabae infection have been characterized. De novo transcriptome assembly provided a total of 39,185 different transcripts that were functionally annotated, and among these, 13,266 were assigned to gene ontology against Arabidopsis. Quality of the assembly was validated by RT-qPCR amplification of selected transcripts differentially expressed. Comparison of faba bean transcripts with those of better-characterized plant genomes such as Arabidopsis thaliana, Medicago truncatula and Cicer arietinum revealed a sequence similarity of 68.3%, 72.8% and 81.27%, respectively. Moreover, 39,060 single nucleotide polymorphism (SNP) and 3,669 InDels were identified for genotyping applications. Mapping of the sequence reads generated onto the assembled transcripts showed that 393 and 457 transcripts were overexpressed in the resistant (29H) and susceptible genotype (Vf136), respectively. Transcripts involved in plant-pathogen interactions such as leucine rich proteins (LRR) or plant growth regulators involved in plant adaptation to abiotic and biotic stresses were found to be differently expressed in the resistant line. The results reported here represent the most comprehensive transcript database developed so far in faba bean, providing valuable information that could be used to gain insight into the pathways involved in the resistance mechanism against A. fabae and to identify potential resistance genes to be further used in marker assisted selection.
This research was supported by the European Community's Seventh Framework Programme (FP7/ 2007-2013) under the grant agreement n°FP7-613551, LEGATO project; the 2014-2020 "Programa Operativo de Crecimiento Inteligente" (RTA2013-00025) and RTA2010-00059, the IPT-2011-1259-010000 project and the P10-CVI-6075, all co-financed by ERDF and the last by the Andalusian regional government.
Peer reviewed
2017-12-21T08:01:44Z
2017-12-21T08:01:44Z
2015-08-12
artículo
http://purl.org/coar/resource_type/c_6501
PLoS ONE 10(8): e0135143 (2015)
1932-6203
http://hdl.handle.net/10261/158427
10.1371/journal.pone.0135143
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011011
26267359
en
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/613551
Publisher's version
http://doi.org/10.1371/journal.pone.0135143
Sí
open
Public Library of Science