2024-03-29T14:31:49Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1570172018-10-03T07:01:44Zcom_10261_9676com_10261_8col_10261_9679
Adaptive TE insertions in Drosophila: NATs miRNAs and piRNAs
González Pérez, Josefa
Ullastres, Ana
Mateo, Lidia
Trabajo presentado en el 14th Congress of the European Society for Evolutionary Biology (ESEB 2013), celebrado en Lisboa del 19 al 24 de agosto de 2013.
Recent Transposable Element (TE) insertions in
Drosophila melanogaster
are a useful tool to identify
adaptive mutations. The objective of this study is moving from the identification of a putatively
adaptive TE insertion to the molecular mechanisms and associated fitness effects. We have focused on
a TE insertion located in the 3'UTR region of
Kmn1
gene. The 3 ́UTR of
Kmn1
overlaps with the 3
́UTR of its nearby gene,
CG11699
, giving rise to a cis-natural antisense pair of transcripts (cis-NATs).
We found that besides being incorporated into the 3’UTR of
Kmn1
, the TE insertion also affects the
transcript length of
CG11699
. Specifically, the TE disrupts the GU-rich downstream element of the
distal polyA signal of
CG11699
and as a consequence a shorter transcript is produced. These structural
changes are very likely to have functional consequences since the presence of the TE adds (
Kmn1
) and
eliminates (
CG11699
) miRNA binding sites, introduces piRNA binding sites (
Kmn1
) and affects the
length of the overlapping region between these two genes. Indeed, we detected that this TE insertion is
associated with an under-expression of
Kmn1
and an over-expression of
CG11699
. In order to identify
the phenotypic effect of this insertion, we first focused on
CG11699
since this gene is involved in the
activation of
Aldh-III
, an enzyme involved in xenobiotic metabolism. We found that the TE insertion is
associated with an increased survival rate after an acute exposure to benzaldehyde, a prototypical drug
classically used to assess
Aldh-III
activity, strongly suggesting that this TE confers resistance to
xenobiotic stress. Our results show that a single mutational event has broad molecular consequences
that can be translated into ecologically relevant phenotypic effects. This example reinforces the idea
that TEs are a powerful natural tool for genome evolution.
No
2017-11-06T11:13:09Z
2017-11-06T11:13:09Z
2013-08
póster de congreso
http://purl.org/coar/resource_type/c_6670
14th Congress of the European Society for Evolutionary Biology (2013)
http://hdl.handle.net/10261/157017
en
Sí
none