2024-03-29T05:51:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1524692021-07-20T11:30:08Zcom_10261_22com_10261_1col_10261_275
Hormonal regulation of malic enzyme and glucose-6-phosphate-dehydrogenaes expression in fetal brown-adipocyte primary cultures under non-proliferative conditions
Valverde, Ángela M.
Benito, Manuel
Lorenzo, Margarita
Consejo Superior de Investigaciones Científicas (España)
Dirección General de Investigación Científica y Técnica, DGICT (España)
The expression of malic enzyme and glucose-6-phosphate (Glc6P) dehydrogenase was investigated in primary cultures of fetal brown adipocytes after the prolonged presence (6 d or 10 d) of various hormones under non-proliferative conditions. The presence of triiodothyronine for 6 d and 10 d resulted in maturation of the triiodothyronine regulatory mechanism of malic-enzyme expression at the mRNA level. However, triiodothyronine had no effect on Glc6P dehydrogenase expression. Insulin increased malic-enzyme and Glc6P dehydrogenase expression at the mRNA and protein level after 6 d and 10 d of culture. The joint presence of triiodothyronine and insulin produced an additive effect on malic-enzyme expression at the mRNA and protein level after 6 d and 10 d of culture, by two independent mechanisms. Noradrenaline prevented the effect at the protein level after 6 d, but not after 10 d, probably due to loss of the β-adrenergic response of brown adipocytes after prolonged culture. Triiodothyronine overexpressed the Glc6P dehydrogenase mRNA induced by the presence of insulin at 6 d and 10 d of culture. There was no adrenergic regulation of Glc6P dehydrogenase expression in cultured fetal brown adipocytes, regardless of the time of culture.
This work was supported by a grant from Dirección General de lnvestigacion Cientifica y Tecnica/Consejo Superior de lnvestigaciones Cientificas, Spain. Angela M. Valverde was a recipient of a fellowship from Consejo Superior de lnvestigaciones Cientificas, Spain.
Peer Reviewed
2017-07-07T08:46:52Z
2017-07-07T08:46:52Z
1992
2017-07-07T08:46:52Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1111/j.1432-1033.1992.tb19861.x
issn: 0014-2956
e-issn: 1432-1033
European Journal of Biochemistry 203(1-2): 313-319 (1992)
http://hdl.handle.net/10261/152469
10.1111/j.1432-1033.1992.tb19861.x
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100008737
Sí
none
John Wiley & Sons