2024-03-28T16:05:17Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1481342018-06-01T04:30:49Zcom_10261_46com_10261_3col_10261_299
Bio-safe processing of polylactic-co-caprolactone and polylactic acid blends to fabricate fibrous porous scaffolds for in vitro mesenchymal stem cells adhesion and proliferation
Salerno, Aurelio
Guarino, Vincenzo
Oliviero, Olimpia
Ambrosio, Luigi
Domingo, Concepción
Generalitat de Catalunya
Ministerio de Economía y Competitividad (España)
Crystallization
Fibrous scaffold
Phase separation
Polymer blend
Stem cells
In this study, the design and fabrication of porous scaffolds, made of blends of polylactic-co-caprolactone (PLC) and polylactic acid (PLA) polymers, for tissue engineering applications is reported. The scaffolds are prepared by means of a bio-safe thermally induced phase separation (TIPS) approach with or without the addition of NaCl particles used as particulate porogen. The scaffolds are characterized to assess their crystalline structure, morphology and mechanical properties, and the texture of the pores and the pore size distribution. Moreover, in vitro human mesenchymal stem cells (hMSCs) culture tests have been carried out to demonstrate the biocompatibility of the scaffolds. The results of this study demonstrate that all of the scaffold materials processed by means of TIPS process are semi-crystalline. Furthermore, the blend composition affected polymer crystallization and, in turn, the nano and macro-structural properties of the scaffolds. Indeed, neat PLC and neat PLA crystallize into globular and randomly arranged sub micro-size scale fibrous conformations, respectively. Concomitantly, the addition of NaCl particles during the fabrication route allows for the creation of an interconnected network of large pores inside the primary structure while resulted in a significant decrease of scaffolds mechanical response. Finally, the results of cell culture tests demonstrate that both the micro and macro-structure of the scaffold affect the in vitro hMSCs adhesion and proliferation.
C. Domingo acknowledges the financial support of the Generalitat de Catalunya through the project 2014SGR-377 and the Spanish Plan Nacional project CTQ2014-56324.
Peer reviewed
2017-04-07T11:15:13Z
2017-04-07T11:15:13Z
2016-06-01
artículo
http://purl.org/coar/resource_type/c_6501
Materials Science and Engineering - C - Materials for Biological Applications 63: 512-521 (2016)
0928-4931
http://hdl.handle.net/10261/148134
10.1016/j.msec.2016.03.018
http://dx.doi.org/10.13039/501100002809
http://dx.doi.org/10.13039/501100003329
en
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-56324-C2-1-P
Postprint
http://dx.doi.org/10.1016/j.msec.2016.03.018
Sí
open
Elsevier