2024-03-29T10:01:51Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1428292022-11-23T12:16:31Zcom_10261_108com_10261_8col_10261_361
Labeling studies on cortical bone formation in the antlers of red deer (Cervus elaphus)
Gómez, Santiago
García, Andrés J.
Luna, Salvador
Gallego, Laureano
Landete-Castillejos, Tomás
Ministerio de Economía y Competitividad (España)
European Commission
Antler
Histology
Osteon
Tetracycline
Mineral apposition rate
The formation and mineralization process of antlers, which constitute the fastest growing bones in vertebrates, is still not fully understood. We used oxytetracycline injections to label different stages of bone formation in antlers of 14 red deer between days 28 and 156 of antler growth. Results show that initially a trabecular scaffold of woven bone is formed which largely replaces a pre-existing scaffold of mineralized cartilage. Lamellar bone is then deposited and from about day 70 onwards, primary osteons fill in the longitudinal tubes lined by the scaffold in a proximal to distal sequence. Mineral apposition rate (MAR) in early stages of primary osteon formation is very high (average 2.15 μm/d). Lower MARs were recorded for later stages of primary osteon formation (1.56 μm/d) and for the smaller secondary osteons (0.89 μm/d). Results suggest a peak in mineral demand around day 100 when the extent of mineralizing surfaces is maximal. A few secondary osteons were formed in a process of antler modeling rather than remodeling, as it occurred simultaneously with formation of primary osteons. The degree of cortical porosity reflects a reduction in MAR during later stages of osteonal growth, whereas cortical thickness is determined earlier. Injections given when the antlers were largely or completely clean from velvet produced no labels in antler bone, strongly suggesting that antlers are dead after velvet shedding. The rapidity of antler mineralization and the short lifespan of antlers make them an extraordinary model to assess the effects of chemicals impairing or promoting bone mineralization.
This research has been partly funded by MEYC-FEDER project CGL2011-24811/BOS and AGL2012-38898, whereas porosity could be measured thanks to the microscope included in project MICINN-FEDER (UNCM08-1E-004).
Peer reviewed
2017-01-20T13:57:32Z
2017-01-20T13:57:32Z
2013
artículo
http://purl.org/coar/resource_type/c_6501
Bone 52(1): 506-515 (2013)
8756-3282
http://hdl.handle.net/10261/142829
10.1016/j.bone.2012.09.015
1873-2763
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
en
http://dx.doi.org/10.1016/j.bone.2012.09.015
Sí
none
Elsevier