2024-03-28T14:49:58Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1425892021-12-27T15:39:46Zcom_10261_125com_10261_2col_10261_378
Characterization of the bile and gall bladder microbiota of healthy pigs
Jiménez, Esther
Sánchez García, Borja
Farina, Annarita
Margolles Barros, Abelardo
Rodríguez Gómez, Juan M.
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
MicrobiologyOpen published by John Wiley & Sons Ltd. Bile is a biological fluid synthesized in the liver, stored and concentrated in the gall bladder (interdigestive), and released into the duodenum after food intake. The microbial populations of different parts of mammal's gastrointestinal tract (stomach, small and large intestine) have been extensively studied; however, the characterization of bile microbiota had not been tackled until now. We have studied, by culture-dependent techniques and a 16S rRNA gene-based analysis, the microbiota present in the bile, gall bladder mucus, and biopsies of healthy sows. Also, we have identified the most abundant bacterial proteins in the bile samples. Our data show that the gall bladder ecosystem is mainly populated by members of the phyla Proteobacteria, Firmicutes, and Bacteroidetes. Furthermore, fluorescent in situ hybridization (FISH) and transmission electron microscopy (TEM) allowed us to visualize the presence of individual bacteria of different morphological types, in close association with either the epithelium or the erythrocytes, or inside the epithelial cells. Our work has generated new knowledge of bile microbial profiles and functions and might provide the basis for future studies on the relationship between bile microbiota, gut microbiota, and health. © 2014 The Authors.
This work was supported by AGL2013-44761-P and AGL2013-41980-P projects from the Ministerio de Ciencia e Innovación (Spain). Borja Sánchez was the recipient of a Ramón y Cajal postdoctoral contract from MINECO.
Peer Reviewed
2017-01-17T10:58:33Z
2017-01-17T10:58:33Z
2014-10-21
2017-01-17T10:58:34Z
artículo
http://purl.org/coar/resource_type/c_6501
issn: 2045-8827
MicrobiologyOpen 3(6): 937-949 (2014)
http://hdl.handle.net/10261/142589
10.1002/mbo3.218
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004837
25336405
Publisher's version
https://doi.org/10.1002/mbo3.218
Sí
open