2024-03-29T14:16:54Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1421932017-04-07T07:17:58Zcom_10261_118com_10261_6col_10261_497
Occurrence of carbon catabolite repression-regulated mechanism involved in the
transport of extracellular ADP-glucose in Escherichia coli
Almagro, Goizeder
Montero, Manuel
Viale, Alejandro M.
Rahimpour, Mehdi
Bahaji, Abdellatif
Muñoz Pérez, Francisco José
Baroja-Fernández, Edurne
Pozueta Romero, Javier
Póster presentado en el XXXVII Congreso de la Sociedad Española de Bioquímica y Biología Molecular (SEBBM), celbrado en Granada del 9 al 12 de septiembre de 2014.
ADP-glucose is the precursor molecule for glycogen and starch biosynthesis
in bacteria and plants, respectively. Due to the high abundance of this
nucleotide-sugar in tubers and endosperms present in the normal diet of
many animals, we explored the possible occurrence of an ADP-glucose
transport system in the enterobacterial species
Escherichia coli.
To avoid artifacts resulting from the possible conversion of periplasmic ADP-glucose
breakdown products into glycogen, in this work we used glglC and pgm
null mutants unable to catalyze the reversible conversion of ADP-glucose
into glucose-1-P, and glucose-1-P into glucose-6-phosphate, respectively.
Noteworthy, these cells converted the exogenoulsy added ADP-glucose into
glycogen. Uptake of ADP-glucose was confirmed by the disappearance of
this nucleotide-sugar from the culture medium.
ΔpgmΔptsG, ΔpgmΔptsH, ΔpgmΔptsI and ΔpgmΔcrr
cells impaired in the PTS components produced
glycogen from exogenously added ADP-glucose, indicating that the
transport of this nucleotide-sugar is not mediated by PTS. Furthermore,
ΔpgmΔcyaA and ΔpgmΔcrp cells, both impaired in the CRP-cAMP carbon
catabolite repression mechanism, neither incorporated ADP-glucose nor
produced glycogen from ADP-glucose, demonstrating that the uptake of
this nucleotide-sugar is CRP-cAMP regulated in
E. coli. Finally, using a
systematic and comprehensive gene-disrupted mutant collection of
E. coli we identifi ed some genes belonging to the CRP-cAMP regulon encoding
membrane proteins whose absence impair ADP-glucose transport.
Peer Reviewed
2017-01-04T12:42:19Z
2017-01-04T12:42:19Z
2014-09
2017-01-04T12:42:19Z
póster de congreso
http://purl.org/coar/resource_type/c_6670
XXXVII Congreso de la Sociedad Española de Bioquímica y Biología Molecular (2014)
http://hdl.handle.net/10261/142193
Sí
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