2024-03-29T01:18:04Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1294472016-06-18T02:27:08Zcom_10261_5063com_10261_5col_10261_5066
Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics
Schulte-Zweckel, Janine
Rosi, Federica
Sreenu, Domalapally
Schröder, Hendrik
Niemeyer, Christof M.
Triola Guillem, Gemma
Amino Acid Motifs
immobilized protein
fluorescent dye
A novel technique for protein immobilization onto CrAsH-modified surfaces is presented. This approach enables an efficient, reversible and fluorogenic immobilization of proteins. Moreover, expressed proteins can also be directly immobilized from cellular lysates without prior purification. The immobilized proteins are suitable for protein-protein interaction studies and the fluorescence enhancement upon immobilization can be employed for the direct detection of the immobilized protein without the need for secondary detection methods.
Peer reviewed
2014-10-28
artículo
http://purl.org/coar/resource_type/c_6501
Chemical Communications
10261/129447
10.1039/c4cc04120h
en
Postprint
DOI: 10.1039/c4cc04120h
Sí
open
Royal Society of Chemistry (UK)