2024-03-19T03:29:41Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1290792021-12-28T16:08:13Zcom_10261_128com_10261_1col_10261_381
Comparative genomics of the Hedgehog loci in chordates and the origins of Shh regulatory novelties
Irimia, Manuel
Royo, José Luis
Burguera, Demian
Maeso, Ignacio
Gómez-Skarmeta, José Luis
Garcia-Fernàndez, Jordi
Consejo Superior de Investigaciones Científicas (España)
European Commission
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Institución Catalana de Investigación y Estudios Avanzados
Junta de Andalucía
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License.
The origin and evolution of the complex regulatory landscapes of some vertebrate developmental genes, often spanning hundreds of Kbp and including neighboring genes, remain poorly understood. The Sonic Hedgehog (Shh) genomic regulatory block (GRB) is one of the best functionally characterized examples, with several discrete enhancers reported within its introns, vast upstream gene-free region and neighboring genes (Lmbr1 and Rnf32). To investigate the origin and evolution of this GRB, we sequenced and characterized the Hedgehog (Hh) loci from three invertebrate chordate amphioxus species, which share several early expression domains with Shh. Using phylogenetic footprinting within and between chordate lineages, and reporter assays in zebrafish probing >30 Kbp of amphioxus Hh, we report large sequence and functional divergence between both groups. In addition, we show that the linkage of Shh to Lmbr1 and Rnf32, necessary for the unique gnatostomate-specific Shh limb expression, is a vertebrate novelty occurred between the two whole-genome duplications.
M.I., D.B., I.M. and J.G.-F. were funded by Grants BFU2005-00252 and BMC2008-03776 and BMC2011-23291 from the Spanish Ministry of Science and Innovation, and J.G.F. by the ICREA Academia Prize. M.I., D.B. and I.M. held FPI, APIF-UB and FPU fellowships, respectively. J.-L.G.-S. and J.L.R. were supported by Grants BFU2010-14839, CSD2007-00008875 (MEC), and CVI 3488 (Junta de Andalucía). JLR holds a JAE-Doc grant from the National Research Council, founded by Social European Funds.
Peer Reviewed
2016-02-15T10:36:57Z
2016-02-15T10:36:57Z
2012
2016-02-15T10:36:59Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1038/srep00433
issn: 2045-2322
Scientific Reports 2: 433 (2012)
http://hdl.handle.net/10261/129079
10.1038/srep00433
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100003741
http://dx.doi.org/10.13039/501100011011
22666536
Publisher's version
http://dx.doi.org/10.1038/srep00433
Sí
open
Nature Publishing Group