2024-03-28T17:00:35Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1280632021-12-27T16:18:48Zcom_10261_79com_10261_1col_10261_332
Peritoneal repairing cells: A type of bone marrow derived progenitor cells involved in mesothelial regeneration
Carmona, R.
Cano, Eva
Grueso, E.
Ruiz-Villalba, A
Bera, T. K.
Gaztambide, J.
Segovia, José Carlos
Muñoz-Chápuli, R.
Ministerio de Ciencia e Innovación (España)
Junta de Andalucía
Instituto de Salud Carlos III
European Commission
Regeneration
Fibrocytes
Bone marrow
Mesothelium
Peritoneum
Progenitor cells
The peritoneal mesothelium exhibits a high regenerative ability. Peritoneal regeneration is concomitant with the appearance, in the coelomic cavity, of a free-floating population of cells whose origin and functions are still under discussion. We have isolated and characterized this cell population and we have studied the process of mesothelial regeneration through flow cytometry and confocal microscopy in a murine model lethally irradiated and reconstituted with GFP-expressing bone marrow cells. In unoperated control mice, most free cells positive for mesothelin, a mesothelial marker, are green fluorescent protein (GFP). However, 24 hrs after peritoneal damage, free mesothelin+/ GFP+ cells appear in peritoneal lavages. Cultured lavage peritoneal cells show colocalization of GFP with mesothelial (mesothelin, cytokeratin) and fibroblastic markers. Immunohistochemical staining of the peritoneal wall also revealed colocalization of GFP with mesothelial markers and with procollagen-1 and smooth muscle α-actin. This was observed in the injured area as well as in the surrounding not-injured peritoneal surfaces. These cells, which we herein call peritoneal repairing cells (PRC), are very abundant 1 week after surgery covering both the damaged peritoneal wall and the surrounding uninjured area. However, they become very scarce 1 month later, when the mesothelium has completely healed. We suggest that PRC constitute a type of monocyte-derived cells, closely related with the tissue-repairing cells known as 'fibrocytes' and specifically involved in peritoneal reparation. Thus, our results constitute a synthesis of the different scenarios hitherto proposed about peritoneal regeneration, particularly recruitment of circulating progenitor cells and adhesion of free-floating coelomic cells. © 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
(Ministerio de Ciencia e Innovación), RD06/0010/0015 (TerCel network, ISCIII), P06-CTS-01614, P08-CTS-03618 (Junta de AndalucÌa) and LSHM-CT-2005–018630 (VI framework, UE)
Peer Reviewed
2016-01-25T13:00:02Z
2016-01-25T13:00:02Z
2011
2016-01-25T13:00:03Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1111/j.1582-4934.2010.01087.x
issn: 1582-1838
Journal of Cellular and Molecular Medicine 15: 1200- 1209 (2011)
http://hdl.handle.net/10261/128063
10.1111/j.1582-4934.2010.01087.x
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011011
20477904
Publisher's version
Sí
open
Blackwell Publishing