2024-03-29T01:42:39Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1164482021-05-12T12:41:53Zcom_10261_105com_10261_1col_10261_358
Administration of nitric oxide with caspase inhibitors minimizes bacterial translocation in experimental intestinal transplantation
Azuara, Daniel
Solà, Anna M.
Hotter, Georgina
Calatayud, Laura
Jaurrieta, Eduardo
Oca, Javier de
Background. Bacterial translocation (BT) has been suggested to be responsible for the high incidence of infections occurring after small-bowel transplantation (Trp). Nitric oxide (NO) and apoptosis could affect cell demise. The aim of this study was to asses whether supplementation of University of Wisconsin (UW) solution with NO donors and apoptosis inhibitors can abolish BT in Trp. Methods. The following experimental groups were studied: sham, Trp, intestinal transplantation, Trp+spermine NONOate (NONOs), and Trp+NONOs+caspase inhibitor Z-Val-Ala-Asp(Ome)-fluoromethylketone (Z-VAD-fmk). Histologic analysis, caspase-3 activity, DNA fragmentation, and BT from graft to mesenteric lymph nodes, liver, and spleen were measured in tissue samples after transplantation. Results. During intestinal transplantation, apoptosis and necrosis were increased, showing graft injury and high levels of BT. The rats treated with NONOs showed a histologic protection of transplanted graft and a decrease in BT despite caspase-3 and DNA fragmentation-inducing effects. Administration of caspase inhibitor Z-VAD to NONOs-treated rats reversed the NO apoptosis-inducing effects and showed the lowest levels of BT in all tissues. Conclusions. Exogenous administration of NO associated with the inhibition of apoptosis maintains the graft in optimal conditions in terms of BT and improves the histology of the graft after intestinal transplantation in rats.
Peer Reviewed
2015-06-11T11:43:17Z
2015-06-11T11:43:17Z
2004
2015-06-11T11:43:17Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1097/01.TP.0000092956.78811.8B
issn: 0041-1337
Transplantation 77(2): 177-183 (2004)
http://hdl.handle.net/10261/116448
10.1097/01.TP.0000092956.78811.8B
http://dx.doi.org/10.1097/01.TP.0000092956.78811.8B
none
Lippincott Williams & Wilkins