2024-03-28T22:05:34Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1130152022-06-01T12:30:09Zcom_10261_105com_10261_1col_10261_358
Effects of the conformationally restricted GABA analogues, cis-and trans-4-aminocrotonic acid, on GABA neurotransmission in primary neuronal cultures
Vale, Carmen
Vilaró, Maria Teresa
Rodríguez-Farré, Eduard
Suñol, Cristina
GABA receptors
Cl- flux
primary neuronal cultures
GABA uptake
RT-PCR
flunitrazepam binding
The effects of the GABA analogues, cis- and trans-4-aminocrotonic acid (ACA) on GABA(A) receptor function and GABA uptake, together with the presence of p-1 subunit mRNA and putative GABA(C) receptors, were studied in primary cultures of neocortical neurons and cerebellar granule cells. Both isomers induced a Cl- influx, which was inhibited by bicuculline, t- butylbicyclophosphorothionate (TBPS), picrotoxinin (PTX), and γ- hexachlorocyclohexane (γ-HCH or lindane). [3H]-flunitrazepam binding was also increased by both isomers and this increase was inhibited by bicuculline. In neocortical neurons, the trans-isomer completely inhibited the [3H]GABA uptake, whereas the cis-isomer produced only a 25% inhibition at the highest concentration used. The possible presence of GABA(C) receptors was investigated only in neocortical cultures by using RT-PCR in order to detect the presence of the mRNA encoding the p-1 subunit which assembles to form homooligomeric Cl- channels. The results presented here show that p-1 subunits, and thus GABA(C) receptors, may represent a very minor population of GABA receptors in these neuronal preparations. We conclude that both GABA analogues may act as agonists at the GABA(A) receptors, although with very different potencies.
Contract grant sponsor: Fondo de Investigación Sanitaria; Contract grant number: 97/0656; Contract grant sponsor: CIRIT, Generalitat de Catalunya; Contract grant numbers: SGR 95-00445, 9500551; Contract grant sponsor: CICYT; Contract grant number: SAF 96-0336
Peer Reviewed
2015-03-26T14:46:50Z
2015-03-26T14:46:50Z
1999-07-01
2015-03-26T14:46:50Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1002/(SICI)1097-4547(19990701)57:1<95::AID-JNR10>3.0.CO;2-N
issn: 0360-4012
Journal of Neuroscience Research 57(1): 95-105 (1999)
http://hdl.handle.net/10261/113015
10.1002/(SICI)1097-4547(19990701)57:1<95::AID-JNR10>3.0.CO;2-N
http://dx.doi.org/10.1002/(SICI)1097-4547(19990701)57:1<95::AID-JNR10>3.0.CO;2-N
none
John Wiley & Sons