2024-03-29T07:40:57Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1124832021-12-28T15:35:15Zcom_10261_41com_10261_1col_10261_294
The Drosophila transcription factor tramtrack (TTK) interacts with Trithorax-like (GAGA) and responsses GAGA-mediated activation
Pagans, Sara
Ortiz-Lombardía, Miguel
Espinàs, Maria Lluïsa
Bernués, Jordi
Azorín, Ferran
In this study, we report the interaction of the Drosophila transcription factors Trithorax-like (GAGA) and tramtrack (TTK). This interaction is documented both in vitro, through GST pull-down assays, as well as in vivo, in yeast and Schneider S2 cells. GAGA and TTK share in common the presence of an N-terminal POZ/BTB domain that was found to be necessary and sufficient for GAGA-TTK interaction. Structural models that could account for this interaction are discussed. GAGA is known to activate the expression of many genes in Drosophila. On the other hand, TTK was proposed to act as a maternally provided repressor of several pair-rule genes, such as even-skipped (eve). As with many Drosophila genes, eve contains at its promoter region binding sites for GAGA and TTK. Here, in transient expression experiments, we showed that GAGA activates transcription from the eve stripe 2 promoter element and that TTK inhibits this GAGA-dependent activation. Repression by TTK of the eve promoter requires its activation by GAGA and depends on the presence of the POZ/BTB domains of TTK and GAGA. These results indicate that GAGA-TTK interaction contributes to the regulation of gene expression in Drosophila.
This work was supported by grants from the Ministerio de Ciencia y Tecnología (BMC2000-878 and BMC2000-898) and the CIRIT (SGR99-185). S.P. and M.O.-L. were recipients of a doctoral fellowship from the CIRIT and a long-term postdoctoral fellowship from FEBS, respectively. This work was carried out within the framework of the `Centre de Referència en Biotecnologia' of the Generalitat de Catalunya
Peer Reviewed
2015-03-17T11:03:09Z
2015-03-17T11:03:09Z
2002
2015-03-17T11:03:09Z
artículo
http://purl.org/coar/resource_type/c_6501
issn: 0305-1048
Nucleic Acids Research 30(20): 4406-4413 (2002)
http://hdl.handle.net/10261/112483
10.1093/nar/gkf570
12384587
Publisher's version
http://dx.doi.org/10.1093/nar/gkf570
open
Oxford University Press