2024-03-29T05:17:06Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/892532020-01-13T13:21:47Zcom_10261_56com_10261_3col_10261_309
Mata, Ana de la
Nieto-Miguel, Teresa
López-Paniagua, Marina
Galindo, Sara
Aguilar, María Rosa
García-Fernández, Luis
Gonzalo, Sandra
Vázquez Lasa, Blanca
San Román, Julio
Corrales, Rosa María
Calonge, Margarita
2014-01-10T11:45:17Z
2014-01-10T11:45:17Z
2013
Journal of Materials Science: Materials in Medicine 24: 2819- 2829 (2013)
http://hdl.handle.net/10261/89253
10.1007/s10856-013-5013-3
The aim of this work was to evaluate semi-synthetic biopolymers based on chitosan (CH) and gelatin (G) as potential in vitro carrier substrata for human limbal epithelial cells (hLECs). To that end, human corneal epithelial cells (HCE) were cultured onto different CH-G membranes. None of the polymers were cytotoxic and cell proliferation was higher when CH was functionalized with G. Expression levels of corneal epithelial markers (K3, K12, E-caherin, desmoplakin, and zonula occludens (ZO)-1) were better maintained in HCE cells grown on CH-G 20:80 membranes than other proportions. Consequently, CH-G 20:80 was chosen for the subsequent expansion of hLECs. Cells derived from limbal explants were successfully expanded on CH-G 20:80 membranes using a culture medium lacking components of non-human animal origin. The expression levels found for corneal (K3 and K12) and limbal epithelial stem cells (K15) specific markers were similar to or higher than those found in limbal cells grown onto the control substratum. Our results demonstrate that CH-G 20:80 membranes are suitable for the expansion and maintenance of stem cells derived from the limbal niche. These results strongly support the use of polymers as alternative substrata for the transplantation of cultivated limbal cells onto the ocular surface. © 2013 Springer Science+Business Media New York.
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Chitosan-gelatin biopolymers as carrier substrata for limbal epithelial stem cells
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