2024-03-28T14:19:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/63262008-10-02T09:44:27Zcom_10261_94com_10261_8com_10261_14col_10261_347col_10261_267
Pendón, Carlos
Martínez-Barberá, Juan P.
Pérez-Sánchez, Jaume
Rodríguez Martínez, Ramón B.
Grenett, Hernán
Valdivia, Manuel M.
2008-08-04T09:23:08Z
2008-08-04T09:23:08Z
1994-08
Gene 145(2): 237-240 (1994)
0378-1119
http://hdl.handle.net/10261/6326
10.1016/0378-1119(94)90012-4
We report here the complete nucleotide (nt) sequence of a cDNA clone encoding Solea senegalensis growth hormone (sGH) isolated from an expression library prepared from sole pituitary gland poly (A)+ RNA. The library was screened using a flounder GH cDNA. The cDNA sequence containing an insert of 769 nt was found to encode a polypeptide of 203 amino acids (aa), including a signal peptide of 17 aa. The 5′- and 3′-untranslated regions of the message are 17 and 119-nt long, respectively. Northern blot hybridization detected a 0.9-kb RNA species. The sGH cDNA sequence shows homologies of 80.9, 76.9, 73.8 and 64.2% with the GH of tuna, gilthead seabream, flounder and rainbow trout.
eng
closedAccess
Recombinant DNA
Fish
Solea senegalensis
Growth hormone
Evolution
Teleost
Northern blot
Pituitary gland
Cloning of the sole (Solea senegalensis) growth hormone-encoding cDNA
artículo